Triticum vulgare Extract Modulates Protein-Kinase B and Matrix Metalloproteinases 9 Protein Expression in BV-2 Cells: Bioactivity on Inflammatory Pathway Associated with Molecular Mechanism Wound Healing
المؤلفون المشاركون
Dini, Valentina
Janowska, Agata
Loggini, Barbara
Minale, Massimiliano
Grieco, Fabrizia
Riccio, Salvatore
Romanelli, Marco
Funel, Niccola
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-02-27
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Matrix metalloproteinases (MMPs) are a large family of ubiquitously expressed zinc-dependent enzymes with proteolitic activities.
They are expressed in physiological situations and pathological conditions involving inflammatory processes including epithelial to mesenchymal transition (EMT), neuronal injury, and cancer.
There is also evidence that MMPs regulate inflammation in tumor microenvironment, which plays an important role in healing tissue processes.
Looking at both inflammatory and neuronal damages, MMP9 is involved in both processes and their modulation seems to be regulated by two proteins: tumor necrosis factor-alpha (TNF-alpha) and interleukin 6 (IL-6).
However other important genes are involved in molecular regulation of transcription factors, protein-kinase B (AKT), and p65.
In addition, Triticum vulgare extract (TVE) modulated the biological markers associated with inflammatory processes, including p65 protein.
While there are no evidence that TVE might be involved in the biological modulation of other inflammatory marker as AKT, we would like to assess whether TVE is able to (1) modulate phosphorylation of AKT (pAKT) as an early marker of inflammatory process in vitro and (2) affect MMP9 protein expression in an in vitro model.
The BV-2 cells (microglial of mouse) have been used as an in vitro model to simulate both inflammatory and neuronal injury pathologies.
Here, MMP9 seems to be involved in cellular migration through inflammatory marker activation.
We simulate an inflammatory preclinical model treating BV-2 cells with lipopolysaccharide (LPS) to induce proinflammatory activation affecting pAKT and p65 proteins.
TVE is revealed to restore the native expression of AKT and p65.
Additionally, TVE extract modulates also the protein concentration of MMP9.
Nevertheless, immunofluorescence confocal analyses revealed that both AKT and MMP9 are regulated together, synchronously.
This work seems to demonstrate that two important genes can be used to monitor the beginning of an inflammatory process, AKT and MMP9, in which TVE seems able to modulate their expression of inflammation-associated molecules.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Funel, Niccola& Dini, Valentina& Janowska, Agata& Loggini, Barbara& Minale, Massimiliano& Grieco, Fabrizia…[et al.]. 2020. Triticum vulgare Extract Modulates Protein-Kinase B and Matrix Metalloproteinases 9 Protein Expression in BV-2 Cells: Bioactivity on Inflammatory Pathway Associated with Molecular Mechanism Wound Healing. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1191611
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Funel, Niccola…[et al.]. Triticum vulgare Extract Modulates Protein-Kinase B and Matrix Metalloproteinases 9 Protein Expression in BV-2 Cells: Bioactivity on Inflammatory Pathway Associated with Molecular Mechanism Wound Healing. Mediators of Inflammation No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1191611
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Funel, Niccola& Dini, Valentina& Janowska, Agata& Loggini, Barbara& Minale, Massimiliano& Grieco, Fabrizia…[et al.]. Triticum vulgare Extract Modulates Protein-Kinase B and Matrix Metalloproteinases 9 Protein Expression in BV-2 Cells: Bioactivity on Inflammatory Pathway Associated with Molecular Mechanism Wound Healing. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1191611
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1191611
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر