Regulation of Cyclooxygenase 2 by Filifactor alocis in Fibroblastic and Monocytic Cells
المؤلفون المشاركون
Deschner, James
Nietzsche, Sandor
Nokhbehsaim, Marjan
Eick, Sigrun
Nogueira, Andressa Vilas Boas
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-02-03
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Periodontitis is a prevalent chronic inflammatory disease triggered by a synergistic and dysbiotic microbiota present in the oral biofilm.
This in vitro study is aimed at evaluating the regulation of cyclooxygenase (COX)2 expression and production by the periodontopathogen Filifactor alocis in human gingival fibroblastic (HGF-1) and monocytic (THP-1) cells and also at investigating the underlying cellular pathway mechanisms.
HGF-1 and THP-1 cells were exposed either to F.
alocis or to the proinflammatory cytokine tumor necrosis factor alpha (TNFα) for 1 and 2 d to examine the COX2 expression by qPCR.
COX2 protein levels were evaluated by ELISA in F.
alocis-stimulated cells.
Both types of cells were also stimulated with a blocking toll-like receptor (TLR)2 antibody or specific inhibitors against MAPKs.
F.
alocis significantly (p<0.05) increased COX2 at both transcriptional and protein levels in both HGF-1 and THP-1 cells.
Moreover, the stimulatory effect of F.
alocis on COX2 was more pronounced in HGF-1 cells in comparison to THP-1 cells.
F.
alocis upregulated the COX2 expression in a dose-dependent manner in both type cells at 1 d.
TNFα also significantly (p<0.05) increased the COX2 expression in both cells.
After preincubation of HGF-1 and THP-1 cells either with a neutralizing anti-TLR2 antibody or with specific MAPK inhibitors, the F.
alocis-upregulated COX2 expression was significantly (p<0.05) suppressed at 1 d.
Our in vitro study provides original evidence that F.
alocis stimulates COX2 production in fibroblastic and monocytic cells through TLR2 and MAPK mechanisms, suggesting a role of this periodontopathogen in the etiopathogenesis of periodontitis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nokhbehsaim, Marjan& Nogueira, Andressa Vilas Boas& Nietzsche, Sandor& Eick, Sigrun& Deschner, James. 2020. Regulation of Cyclooxygenase 2 by Filifactor alocis in Fibroblastic and Monocytic Cells. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1191727
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nokhbehsaim, Marjan…[et al.]. Regulation of Cyclooxygenase 2 by Filifactor alocis in Fibroblastic and Monocytic Cells. Mediators of Inflammation No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1191727
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nokhbehsaim, Marjan& Nogueira, Andressa Vilas Boas& Nietzsche, Sandor& Eick, Sigrun& Deschner, James. Regulation of Cyclooxygenase 2 by Filifactor alocis in Fibroblastic and Monocytic Cells. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1191727
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1191727
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر