![](/images/graphics-bg.png)
Role of Oxygen Supply in Macrophages in a Model of Simulated Orthodontic Tooth Movement
المؤلفون المشاركون
Deschner, James
Proff, P.
Kirschneck, Christian
Schröder, Agnes
Jantsch, Jonathan
Barschkies, Leonie
Gölz, Lina
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-29
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Apart from periodontal ligament fibroblasts, immune cells like macrophages also play an important mediating role in orthodontic tooth movement (OTM).
Upon orthodontic force application to malpositioned teeth, macrophages in the periodontal ligament get exposed to both mechanical strain and hypoxic conditions (via a compression of blood vessels).
In this study, we assessed the relative impact of orthodontically induced mechanical strain and hypoxic conditions on macrophages for the mediation and regulation of OTM.
Macrophages were stimulated with physiological orthodontic compressive forces of 2 g/cm2 for 4 h and 24 h on gas-impermeable or gas-permeable cell culture plates under normoxic or hypoxic cell culture conditions.
We quantified expression of genes involved in inflammation (Tnf, Il-6, and Cox-2), extracellular remodelling (Mmp-9), and angiogenesis (Vegf) by RT-qPCR.
Furthermore, we analysed HIF-1α, prostaglandin-E2, and VEGF protein expression via immunoblotting or ELISA.
Mechanical strain and oxygen supply both differentially affected expression of genes and proteins involved in inflammation and angiogenesis.
In this context, we found that HIF-1α protein levels were elevated by combined mechanical strain and hypoxic conditions, whereas gas-permeable plates providing sufficient oxygen supply prevented HIF-1α stabilization at the protein level after pressure application on macrophages.
Our results thus indicate that macrophages involved in the mediation of OTM are affected by and respond differently to hypoxic conditions and mechanical compressive strain, which occur concomitantly during OTM, than periodontal ligament fibroblasts (PDLF), thus indicating different roles of these cells in the regulation of OTM at the cellular-molecular level.
We further observed that contrary to PDLF HIF-1α stabilization in macrophages is rather induced via the decreased oxygen supply associated with OTM than via mechanotransduction by mechanical strain.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Schröder, Agnes& Barschkies, Leonie& Jantsch, Jonathan& Proff, P.& Gölz, Lina& Deschner, James…[et al.]. 2020. Role of Oxygen Supply in Macrophages in a Model of Simulated Orthodontic Tooth Movement. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1191839
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Schröder, Agnes…[et al.]. Role of Oxygen Supply in Macrophages in a Model of Simulated Orthodontic Tooth Movement. Mediators of Inflammation No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1191839
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Schröder, Agnes& Barschkies, Leonie& Jantsch, Jonathan& Proff, P.& Gölz, Lina& Deschner, James…[et al.]. Role of Oxygen Supply in Macrophages in a Model of Simulated Orthodontic Tooth Movement. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1191839
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1191839
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)