Advanced Glycated apoA-IV Loses Its Ability to Prevent the LPS-Induced Reduction in Cholesterol Efflux-Related Gene Expression in Macrophages
المؤلفون المشاركون
Machado, Ubiratan Fabres
Passarelli, Marisa
Shimabukuro Okuda, Ligia
Tallada Iborra, Rodrigo
Ramos Pinto, Paula
Corrêa-Giannella, Maria Lucia
Pickford, Russell
Woods, Tom
Brimble, Margaret Anne
Rye, Kerry-Anne
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-14
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
We addressed how advanced glycation (AGE) affects the ability of apoA-IV to impair inflammation and restore the expression of genes involved in cholesterol efflux in lipopolysaccharide- (LPS-) treated macrophages.
Recombinant human apoA-IV was nonenzymatically glycated by incubation with glycolaldehyde (GAD), incubated with cholesterol-loaded bone marrow-derived macrophages (BMDMs), and then stimulated with LPS prior to measurement of proinflammatory cytokines by ELISA.
Genes involved in cholesterol efflux were quantified by RT-qPCR, and cholesterol efflux was measured by liquid scintillation counting.
Carboxymethyllysine (CML) and pyrraline (PYR) levels, determined by Liquid Chromatography-Mass Spectrometry (LC-MS/MS), were greater in AGE-modified apoA-IV (AGE-apoA-IV) compared to unmodified-apoA-IV.
AGE-apoA-IV inhibited expression of interleukin 6 (Il6), TNF-alpha (Tnf), IL-1 beta (Il1b), toll-like receptor 4 (Tlr4), tumor necrosis factor receptor-associated factor 6 (Traf6), Janus kinase 2/signal transducer and activator of transcription 3 (Jak2/Stat3), nuclear factor kappa B (Nfkb), and AGE receptor 1 (Ddost) as well as IL-6 and TNF-alpha secretion.
AGE-apoA-IV alone did not change cholesterol efflux or ABCA-1 levels but was unable to restore the LPS-induced reduction in expression of Abca1 and Abcg1.
AGE-apoA-IV inhibited inflammation but lost its ability to counteract the LPS-induced changes in expression of genes involved in macrophage cholesterol efflux that may contribute to atherosclerosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shimabukuro Okuda, Ligia& Tallada Iborra, Rodrigo& Ramos Pinto, Paula& Machado, Ubiratan Fabres& Corrêa-Giannella, Maria Lucia& Pickford, Russell…[et al.]. 2020. Advanced Glycated apoA-IV Loses Its Ability to Prevent the LPS-Induced Reduction in Cholesterol Efflux-Related Gene Expression in Macrophages. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1191912
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shimabukuro Okuda, Ligia…[et al.]. Advanced Glycated apoA-IV Loses Its Ability to Prevent the LPS-Induced Reduction in Cholesterol Efflux-Related Gene Expression in Macrophages. Mediators of Inflammation No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1191912
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shimabukuro Okuda, Ligia& Tallada Iborra, Rodrigo& Ramos Pinto, Paula& Machado, Ubiratan Fabres& Corrêa-Giannella, Maria Lucia& Pickford, Russell…[et al.]. Advanced Glycated apoA-IV Loses Its Ability to Prevent the LPS-Induced Reduction in Cholesterol Efflux-Related Gene Expression in Macrophages. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1191912
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1191912
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر