The Supernatant of Tonsil-Derived Mesenchymal Stem Cell Has Antiallergic Effects in Allergic Rhinitis Mouse Model
المؤلفون المشاركون
Kim, Dong-Kyu
Bae, Jun-Sang
Mo, Ji-Hun
Park, In-Su
Kim, Ji Hye
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-04-07
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Background and Purpose.
Recently, tonsil-derived mesenchymal stem cells (T-MSCs) have attracted great attention in various medical fields due to easier harvest of T-MSCs and more immunomodulatory effects than adipose-derived MSCs.
However, there was still little evidence of the effect of conditioned media from T-MSCs (T-MSCs-CM) on allergic rhinitis (AR).
Therefore, we investigated the impact of T-MSCs-CM on an AR mouse model.
Methods.
We isolated T-MSCs from human palatine tonsil and evaluated the ingredients of T-MSCs-CM.
The effect of T-MSCs-CM was evaluated in the AR mouse model that was randomly divided into five groups (negative control, positive control, and T-MSCs-CM treated (0.1 mg, 1 mg, and 10 mg)).
To investigate the therapeutic effect, we analyzed rhinitis symptoms, serum immunoglobulin (Ig), inflammatory cells, and cytokine expression.
We also assessed T cell receptor signal, including MAP kinase (ERK/JNK), p65, and NFAT1.
Results.
We identified the increment of TGF-β1, PGE2, and HGF in the T-MSCs-CM.
In an animal study, the T-MSCs-CM-treated group showed significantly reduced allergic symptoms and infiltration of eosinophils and neutrophils in the nasal mucosa, whereas there was no significant difference in total IgE and the OVA-specific IgE level.
Additionally, we found that the 10 mg T-MSCs-CM-treated group showed a significantly decreased IL-4 mRNA expression, compared to the (+) Con group.
In the analysis of T cell receptor signal, the phosphorylation of MAP kinases, translocation of p65, and activation of NFAT1 were inhibited after T-MSCs-CM.
Conclusions.
Our findings suggest that T-MSCs-CM showed a partial immunomodulatory effect on the AR mouse model by the inhibition of T cell activation via MAP kinase, p65, and NFAT1.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Park, In-Su& Kim, Ji Hye& Bae, Jun-Sang& Kim, Dong-Kyu& Mo, Ji-Hun. 2020. The Supernatant of Tonsil-Derived Mesenchymal Stem Cell Has Antiallergic Effects in Allergic Rhinitis Mouse Model. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1191943
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Park, In-Su…[et al.]. The Supernatant of Tonsil-Derived Mesenchymal Stem Cell Has Antiallergic Effects in Allergic Rhinitis Mouse Model. Mediators of Inflammation No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1191943
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Park, In-Su& Kim, Ji Hye& Bae, Jun-Sang& Kim, Dong-Kyu& Mo, Ji-Hun. The Supernatant of Tonsil-Derived Mesenchymal Stem Cell Has Antiallergic Effects in Allergic Rhinitis Mouse Model. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1191943
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1191943
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر