Evaluation of Biophysical Interaction between Newly Synthesized Pyrazoline Pyridazine Derivative and Bovine Serum Albumin by Spectroscopic and Molecular Docking Studies
المؤلفون المشاركون
Bakheit, Ahmed H.
Bhat, Mashooq A.
Wani, Tanveer A.
Zargar, Seema
Al-Mehizia, Abdulrahman A.
Asmari, Majid Mohammed
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-06-16
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
In this research, the pyrazoline pyridazine derivative 7-methyl-2-phenyl-4-(3,4,5-trimethoxyphenyl)-2H-pyrazolo[3,4-d]pyridazine (5d) was studied for its interaction with bovine serum albumin (BSA).
Various spectroscopic techniques along with molecular docking analysis were utilized to understand the mechanism of interaction.
The quenching of BSA fluorescence by using investigational drug 5d was the basic principle for the methodology.
Spectrofluorometric methods and UV-absorption studies were conducted for exploration of the 5d and BSA binding mechanism.
The fluorescence quenching mechanism involved in BSA and 5d interaction was static quenching, and a complex formation also occurred between them.
Both enthalpy and entropy attained positive values suggesting involvement of hydrophobic forces in BSA and 5d interaction.
The Förster distance of 2.23 nm was calculated by fluorescence resonance energy transfer (FRET).
An alteration in BSA secondary structure was proven from the conformational studies of BSA-5d interaction.
This binding interaction study provided a basis to comprehend the binding interaction between 5d and BSA.
These results provided information about sites of BSA involved in its interaction with 5d.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Al-Mehizia, Abdulrahman A.& Bakheit, Ahmed H.& Zargar, Seema& Bhat, Mashooq A.& Asmari, Majid Mohammed& Wani, Tanveer A.. 2019. Evaluation of Biophysical Interaction between Newly Synthesized Pyrazoline Pyridazine Derivative and Bovine Serum Albumin by Spectroscopic and Molecular Docking Studies. Journal of Spectroscopy،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1192031
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Al-Mehizia, Abdulrahman A.…[et al.]. Evaluation of Biophysical Interaction between Newly Synthesized Pyrazoline Pyridazine Derivative and Bovine Serum Albumin by Spectroscopic and Molecular Docking Studies. Journal of Spectroscopy No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1192031
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Al-Mehizia, Abdulrahman A.& Bakheit, Ahmed H.& Zargar, Seema& Bhat, Mashooq A.& Asmari, Majid Mohammed& Wani, Tanveer A.. Evaluation of Biophysical Interaction between Newly Synthesized Pyrazoline Pyridazine Derivative and Bovine Serum Albumin by Spectroscopic and Molecular Docking Studies. Journal of Spectroscopy. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1192031
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1192031
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر