ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes
المؤلفون المشاركون
Huang, Shan-shan
Fang, Pei-pei
Pan, Chen-wei
Zhou, Guang-yao
Du, Wen-jun
Li, Qiang
Li, Jie
Lin, Wei
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-09
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Background.
Apoptosis signal-regulating kinase 1 (ASK1) has been reported to induce fibrotic signaling in the setting of oxidative stress.
However, the role of ASK1 and its mechanism of action in angiotensin II- (Ang II-) induced liver fibrosis remain largely unknown.
Methods.
Human hepatic LX-2 stellate cells were treated with Ang II alone or cotreated with Ang II plus an ASK1 inhibitor (GS-4997) or siRNA-targeting ASK1.
Immunofluorescent staining, real-time PCR, and western blotting were used to determine the expressionof α-SMA, Col I, and Col III expression.
Cell viability was assessed by the CCK-8 assay.
The concentrations of IL-1β, IL-18, and TNF-α in conditioned medium were determined by ELISA.
The levels of intracellular ROS in LX-2 cells were analyzed using a ROS assay kit.
Exosome size was determined by electron microscopy.
Results.
Ang II markedly increased the expression of extracellular matrix (ECM) proteins (α-SMA, Col I, and Col III) and proinflammatory cytokines (IL-1β, IL-18, and TNF-α).
Ang II also increased the expression of endoplasmic reticulum stress (ERS) markers (GRP78, p-PERK, and CHOP) and p-ASK1.
Results also showed that pretreatment with GS-4997 or siRNA could abolish all the abovementioned effects on LX-2 cells.
Furthermore, we found that exosome release caused by ASK1-mediated ERS was involved in the activation of LX-2 cells by Ang II.
The activation of LX-2 cells could be blocked by treating the exosomes with annexin.
Conclusions.
In summary, we found that ASK1 mediates Ang II-activated ERS in HSCs and the subsequent activation of HSCs, suggesting a promising strategy for treating liver fibrosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fang, Pei-pei& Pan, Chen-wei& Lin, Wei& Li, Jie& Huang, Shan-shan& Zhou, Guang-yao…[et al.]. 2020. ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1192032
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fang, Pei-pei…[et al.]. ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes. Mediators of Inflammation No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1192032
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fang, Pei-pei& Pan, Chen-wei& Lin, Wei& Li, Jie& Huang, Shan-shan& Zhou, Guang-yao…[et al.]. ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1192032
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1192032
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر