Methotrexate Combined with 4-Hydroperoxycyclophosphamide Downregulates Multidrug-Resistance P-Glycoprotein Expression Induced by Methotrexate in Rheumatoid Arthritis Fibroblast-Like Synoviocytes via the JAK2STAT3 Pathway
المؤلفون المشاركون
Wang, Caihong
Li, Xiaofeng
Gao, Chong
Qin, Kaili
Chen, Kailin
Zhao, Wenpeng
Wang, Qun
Luo, Jing
Zhao, Xiangcong
المصدر
Journal of Immunology Research
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-02-18
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Objective.
Rheumatoid arthritis (RA) multidrug resistance is associated with P-glycoprotein (P-gp) overexpression.
We investigated the effects of methotrexate (MTX) alone and combined with 4-hydroperoxycyclophosphamide (4-HC) on P-gp expression in fibroblast-like synoviocytes (FLSs) from patients with RA and examined the signaling pathway involved.
Methods.
RA-FLSs were treated with MTX, MTX + 4-HC, AG490 + MTX, or AG490 + MTX + 4-HC for 72 h.
Proliferation inhibition rates were determined by MTT assay; P-gp expression was measured by flow cytometry and real-time polymerase chain reaction (RT-PCR); JAK2 and STAT3 were measured by RT-PCR and cell-based ELISA to assess STAT3 signaling.
Results.
MTX alone significantly induced P-gp expression and mRNA production in RA-FLSs.
P-gp expression and mRNA levels were lower in the MTX + 4-HC group than in the MTX-alone group.
In contrast to MTX, MTX + 4-HC reduced the STAT3 phosphorylation and downregulated JAK2 and STAT3 mRNA production.
Inhibition of constitutively active STAT3 accompanied by 4-HC suppressed P-gp levels in RA-FLSs.
The MTT assays revealed no significant differences in proliferation inhibition rates among groups.
Conclusions.
The increased anti-P-gp effect of MTX + 4-HC versus MTX alone in RA-FLSs was mediated via inhibition of the JAK2/STAT3 pathway and may have helped reverse MDR in refractory RA patients with high-P-gp levels.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Qin, Kaili& Chen, Kailin& Zhao, Wenpeng& Zhao, Xiangcong& Luo, Jing& Wang, Qun…[et al.]. 2018. Methotrexate Combined with 4-Hydroperoxycyclophosphamide Downregulates Multidrug-Resistance P-Glycoprotein Expression Induced by Methotrexate in Rheumatoid Arthritis Fibroblast-Like Synoviocytes via the JAK2STAT3 Pathway. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1192118
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Qin, Kaili…[et al.]. Methotrexate Combined with 4-Hydroperoxycyclophosphamide Downregulates Multidrug-Resistance P-Glycoprotein Expression Induced by Methotrexate in Rheumatoid Arthritis Fibroblast-Like Synoviocytes via the JAK2STAT3 Pathway. Journal of Immunology Research No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1192118
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Qin, Kaili& Chen, Kailin& Zhao, Wenpeng& Zhao, Xiangcong& Luo, Jing& Wang, Qun…[et al.]. Methotrexate Combined with 4-Hydroperoxycyclophosphamide Downregulates Multidrug-Resistance P-Glycoprotein Expression Induced by Methotrexate in Rheumatoid Arthritis Fibroblast-Like Synoviocytes via the JAK2STAT3 Pathway. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1192118
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1192118
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر