Combinational Pretreatment of Colony-Stimulating Factor 1 Receptor Inhibitor and Triptolide Upregulates BDNF-Akt and Autophagic Pathways to Improve Cerebral Ischemia
المؤلفون المشاركون
Gao, Feng
Du, Xiaoxue
Chen, Shijia
Botchway, Benson O. A.
Amin, Nashwa
Fang, Marong
Hu, Zhiying
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-10-31
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Ki20227, a selective inhibitor of colony-stimulating factor 1 receptor (CSF1R), has been suggested to regulate microglia inflammatory function and neuronal synaptic plasticity.
Triptolide (TP) pretreatment has neuroprotective effects through its anti-inflammatory and antiapoptotic features in ischemic stroke mice.
However, the underlying mechanism and pathway are presently unclear.
We thus investigated the association between neuroprotective effects of combined TP and Ki20227 and BDNF-Akt and autophagy pathways.
Ki20227 was administrated for 7 days, and TP was administered once 24 hours prior to building the ischemic stroke model in C57BL/6 mice.
Behavioral tests, Golgi staining, immunofluorescence, and western blot analyses were employed to examine neuroprotective effects of TP and Ki20227.
TP and Ki20227 pretreatments improved the neurobehavioral function in stroke mice.
Synaptic protein expressions and density of dendritic spine density were upregulated in Ki20227 and TP pretreated stroke mice.
Further, optimized integration of TP and Ki20227 pretreatments upregulated the NeuN expression and downregulated Iba1 expression after stroke.
In addition, both TP and Ki20227 pretreatments significantly upregulated BDNF, p-Akt/Akt, and Erk1/2 protein expressions and autophagy related proteins (LC3II/I, Atg5, and p62), indicating the activation of BDNF and autophagic pathways.
Optimized integration of TP and Ki20227 can improve cerebral ischemia by inhibiting CSF1R signal and trigger autophagy and BDNF-Akt signaling pathways to increase dendritic spine density and synaptic protein expressions, which in turn enhances neurobehavioral function.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Du, Xiaoxue& Gao, Feng& Chen, Shijia& Botchway, Benson O. A.& Amin, Nashwa& Hu, Zhiying…[et al.]. 2020. Combinational Pretreatment of Colony-Stimulating Factor 1 Receptor Inhibitor and Triptolide Upregulates BDNF-Akt and Autophagic Pathways to Improve Cerebral Ischemia. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1192136
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Du, Xiaoxue…[et al.]. Combinational Pretreatment of Colony-Stimulating Factor 1 Receptor Inhibitor and Triptolide Upregulates BDNF-Akt and Autophagic Pathways to Improve Cerebral Ischemia. Mediators of Inflammation No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1192136
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Du, Xiaoxue& Gao, Feng& Chen, Shijia& Botchway, Benson O. A.& Amin, Nashwa& Hu, Zhiying…[et al.]. Combinational Pretreatment of Colony-Stimulating Factor 1 Receptor Inhibitor and Triptolide Upregulates BDNF-Akt and Autophagic Pathways to Improve Cerebral Ischemia. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1192136
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1192136
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر