Carbamazepine-Mediated Adverse Drug Reactions: CBZ-10,11-epoxide but Not Carbamazepine Induces the Alteration of Peptides Presented by HLA-B∗15:02
المؤلفون المشاركون
Blasczyk, Rainer
Kunze-Schumacher, Heike
Bade-Doeding, Christina
Huyton, Trevor
Celik, Alexander A.
Kuhn, Joachim
Simper, Gwendolin S.
Hò, Gia-Gia T.
المصدر
Journal of Immunology Research
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-09-13
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Among patients treated with the anticonvulsive and psychotropic drug carbamazepine (CBZ), approximately 10% develop severe and life-threatening adverse drug reactions.
These immunological conditions are resolved upon withdrawal of the medicament, suggesting that the drug does not manifest in the body in long term.
The HLA allele B∗15:02 has been described to be a genomic biomarker for CBZ-mediated immune reactions.
It is not well understood if the immune reactions are triggered by the original drug or by its metabolite carbamazepine-10,11-epoxide (EPX) and how the interaction between the drug and the distinct HLA molecule occurs.
Genetically engineered human B-lymphoblastoid cells expressing soluble HLA-B∗15:02 molecules were treated with the drug or its metabolite.
Functional pHLA complexes were purified; peptides were eluted and sequenced.
Applying mass spectrometric analysis, CBZ and EPX were monitored by analyzing the heavy chain and peptide fractions separately for the presence of the drug.
This method enabled the detection of the drug in a biological situation post-pHLA assembly.
Both drugs were bound to the HLA-B∗15:02 heavy chain; however, solely EPX altered the peptide-binding motif of B∗15:02-restricted peptides.
This observation could be explained through structural insight; EPX binds to the peptide-binding region and alters the biochemical features of the F pocket and thus the peptide motif.
Understanding the nature of immunogenic interactions between CBZ and EPX with the HLA immune complex will guide towards effective and safe medications.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Simper, Gwendolin S.& Hò, Gia-Gia T.& Celik, Alexander A.& Huyton, Trevor& Kuhn, Joachim& Kunze-Schumacher, Heike…[et al.]. 2018. Carbamazepine-Mediated Adverse Drug Reactions: CBZ-10,11-epoxide but Not Carbamazepine Induces the Alteration of Peptides Presented by HLA-B∗15:02. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1192477
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Simper, Gwendolin S.…[et al.]. Carbamazepine-Mediated Adverse Drug Reactions: CBZ-10,11-epoxide but Not Carbamazepine Induces the Alteration of Peptides Presented by HLA-B∗15:02. Journal of Immunology Research No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1192477
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Simper, Gwendolin S.& Hò, Gia-Gia T.& Celik, Alexander A.& Huyton, Trevor& Kuhn, Joachim& Kunze-Schumacher, Heike…[et al.]. Carbamazepine-Mediated Adverse Drug Reactions: CBZ-10,11-epoxide but Not Carbamazepine Induces the Alteration of Peptides Presented by HLA-B∗15:02. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1192477
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1192477
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر