Cytokines and Chemokines in Pediatric Appendicitis: A Multiplex Analysis of Inflammatory Protein Mediators
المؤلفون المشاركون
Thompson, Graham Cameron
Naqvi, S. Ali
Barkema, Herman W.
Jenne, Craig N.
Joffe, Ari R.
Blackwood, Jaime
Martin, Dori-Ann
Brindle, Mary
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-02-21
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Objectives.
We aimed to demonstrate the potential of precision medicine to describe the inflammatory landscape present in children with suspected appendicitis.
Our primary objective was to determine levels of seven inflammatory protein mediators previously associated with intra-abdominal inflammation (C-reactive protein—CRP, procalcitonin—PCT, interleukin-6 (IL), IL-8, IL-10, monocyte chemoattractant protein-1—MCP-1, and serum amyloid A—SAA) in a cohort of children with suspected appendicitis.
Subsequently, using a multiplex proteomics approach, we examined an expansive array of novel candidate cytokine and chemokines within this population.
Methods.
We performed a secondary analysis of targeted proteomics data from Alberta Sepsis Network studies.
Plasma mediator levels, analyzed by Luminex multiplex assays, were evaluated in children aged 5-17 years with nonappendicitis abdominal pain (NAAP), acute appendicitis (AA), and nonappendicitis sepsis (NAS).
We used multivariate regression analysis to evaluate the seven target proteins, followed by decision tree and heat mapping analyses for all proteins evaluated.
Results.
185 children were included: 83 with NAAP, 79 AA, and 23 NAS.
Plasma levels of IL-6, CRP, MCP-1, PCT, and SAA were significantly different in children with AA compared to those with NAAP (p<0.001).
Expansive proteomic analysis demonstrated 6 patterns in inflammatory mediator profiles based on severity of illness.
A decision tree incorporating the proteins CRP, ferritin, SAA, regulated on activation normal T-cell expressed and secreted (RANTES), monokine induced by gamma interferon (MIG), and PCT demonstrated excellent specificity (0.920) and negative predictive value (0.882) for children with appendicitis.
Conclusions.
Multiplex proteomic analyses described the inflammatory landscape of children presenting to the ED with suspected appendicitis.
We have demonstrated the feasibility of this approach to identify potential novel candidate cytokines/chemokine patterns associated with a specific illness (appendicitis) amongst those with a broad ED presentation (abdominal pain).
This approach can be modelled for future research initiatives in pediatric emergency medicine.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Naqvi, S. Ali& Thompson, Graham Cameron& Joffe, Ari R.& Blackwood, Jaime& Martin, Dori-Ann& Brindle, Mary…[et al.]. 2019. Cytokines and Chemokines in Pediatric Appendicitis: A Multiplex Analysis of Inflammatory Protein Mediators. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1192700
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Naqvi, S. Ali…[et al.]. Cytokines and Chemokines in Pediatric Appendicitis: A Multiplex Analysis of Inflammatory Protein Mediators. Mediators of Inflammation No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1192700
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Naqvi, S. Ali& Thompson, Graham Cameron& Joffe, Ari R.& Blackwood, Jaime& Martin, Dori-Ann& Brindle, Mary…[et al.]. Cytokines and Chemokines in Pediatric Appendicitis: A Multiplex Analysis of Inflammatory Protein Mediators. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1192700
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1192700
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر