METTL3 Attenuates LPS-Induced Inflammatory Response in Macrophages via NF-κB Signaling Pathway
المؤلفون المشاركون
Xu, Donghua
Lu, Hongying
Wang, Jinghua
Wang, Shufeng
Yan, Shushan
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-10-24
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Methyltransferase-like 3 (METTL3), an RNA N6-methyladenosine (m6A) methyltransferase, is essential for the m6A mRNA modification.
As a key enzyme of m6A methylation modification, METTL3 has been implicated in immune and inflammation regulation.
However, little is known of the role and underlying mechanism of METTL3 in rheumatoid arthritis (RA).
The aim of the present study is to elucidate the function and potential mechanism of METTL3 in RA pathogenesis.
We used quantitative real-time polymerase chain reaction to detect the expression of METTL3 in RA patients and controls as well as the macrophage cell line.
CCK-8 was used for cell proliferation assay.
Enzyme-linked immunosorbent assay (ELISA) was adopted to estimate the generation of IL-6 and TNF-α in macrophages.
Western blot and immunofluorescence were applied to evaluate the activation of NF-κB in macrophages.
The expression of METTL3 was significantly elevated in patients with RA.
It was positively associated with CRP and ESR, two common markers for RA disease activity.
Besides, LPS could enhance the expression and biological activity of METTL3 in macrophages, while overexpression of METTL3 significantly attenuated the inflammatory response induced by LPS in macrophages.
Moreover, the effect of METTL3 on LPS-induced inflammation in macrophages was dependent on NF-κB.
This study firstly demonstrates the critical role of METTL3 in RA, which provides novel insights into recognizing the pathogenesis of RA and a promising biomarker for RA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Jinghua& Yan, Shushan& Lu, Hongying& Wang, Shufeng& Xu, Donghua. 2019. METTL3 Attenuates LPS-Induced Inflammatory Response in Macrophages via NF-κB Signaling Pathway. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1192762
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Jinghua…[et al.]. METTL3 Attenuates LPS-Induced Inflammatory Response in Macrophages via NF-κB Signaling Pathway. Mediators of Inflammation No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1192762
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Jinghua& Yan, Shushan& Lu, Hongying& Wang, Shufeng& Xu, Donghua. METTL3 Attenuates LPS-Induced Inflammatory Response in Macrophages via NF-κB Signaling Pathway. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1192762
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1192762
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر