The cAMP Pathway Amplifies Early MyD88-Dependent and Type I Interferon-Independent LPS-Induced Interleukin-10 Expression in Mouse Macrophages
المؤلفون المشاركون
Ernst, Orna
Glucksam-Galnoy, Yifat
Athamna, Muhammad
Ben-Dror, Iris
Ben-Arosh, Hadar
Levy-Rimler, Galit
Fraser, Iain D. C.
Zor, Tsaffrir
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-17
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Interleukin-10 (IL-10) is a key anti-inflammatory cytokine, secreted by macrophages and other immune cells to attenuate inflammation.
Autocrine type I interferons (IFNs) largely mediate the delayed expression of IL-10 by LPS-stimulated macrophages.
We have previously shown that IL-10 is synergistically expressed in macrophages following a costimulus of a TLR agonist and cAMP.
We now show that the cAMP pathway directly upregulates IL-10 transcription and plays an important permissive and synergistic role in early, but not late, LPS-stimulated IL-10 mRNA and protein expression in mouse macrophages and in a mouse septic shock model.
Our results suggest that the loss of synergism is not due to desensitization of the cAMP inducing signal, and it is not mediated by a positive crosstalk between the cAMP and type I IFN pathways.
First, cAMP elevation in LPS-treated cells decreased the secretion of type I IFN.
Second, autocrine/paracrine type I IFNs induce IL-10 promoter reporter activity only additively, but not synergistically, with the cAMP pathway.
IL-10 promoter reporter activity was synergistically induced by cAMP elevation in macrophages stimulated by an agonist of either TLR4, TLR2/6, or TLR7, receptors which signal via MyD88, but not by an agonist of TLR3 which signals independently of MyD88.
Moreover, MyD88 knockout largely reduced the synergistic IL-10 expression, indicating that MyD88 is required for the synergism displayed by LPS with cAMP.
This report delineates the temporal regulation of early cAMP-accelerated vs.
late type I IFN-dependent IL-10 transcription in LPS-stimulated murine macrophages that can limit inflammation at its onset.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ernst, Orna& Glucksam-Galnoy, Yifat& Athamna, Muhammad& Ben-Dror, Iris& Ben-Arosh, Hadar& Levy-Rimler, Galit…[et al.]. 2019. The cAMP Pathway Amplifies Early MyD88-Dependent and Type I Interferon-Independent LPS-Induced Interleukin-10 Expression in Mouse Macrophages. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1192810
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ernst, Orna…[et al.]. The cAMP Pathway Amplifies Early MyD88-Dependent and Type I Interferon-Independent LPS-Induced Interleukin-10 Expression in Mouse Macrophages. Mediators of Inflammation No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1192810
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ernst, Orna& Glucksam-Galnoy, Yifat& Athamna, Muhammad& Ben-Dror, Iris& Ben-Arosh, Hadar& Levy-Rimler, Galit…[et al.]. The cAMP Pathway Amplifies Early MyD88-Dependent and Type I Interferon-Independent LPS-Induced Interleukin-10 Expression in Mouse Macrophages. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1192810
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1192810
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر