Abnormal Expression of DICER1 Leads to Dysregulation of Inflammatory Effectors in Human Synoviocytes
المؤلفون المشاركون
Jiang, Congshan
Xu, Jing
Zhu, Wenhua
Cai, Yongsong
Wang, Si
Guo, Yuanxu
Xu, Ke
Geng, Manman
Hussain, Nazim
Han, Yan
Zhang, Fujun
Ning, Qilan
Meng, Liesu
Lu, Shemin
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-05-28
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Dysregulation of multiple microRNAs widely takes place during rheumatoid arthritis (RA) and experimental arthritides.
This study is performed to explore the possible mechanism underlying DICER1 deficiency-mediated inflammation in human synoviocytes SW982.
Firstly, RNAi of DICER1 led to increased COX2, MMP3, and MMP13 protein production, while DICER1 overexpression could reduce MMP13 expression.
Secondly, the increase of IL-8 and decrease of TGF-β1 and TIMP1 were determined in the supernatant derived from DICER1 siRNA-treated cells, while DICER1 overexpression was found capable to reverse this effect.
Ingenuity pathway analysis (IPA) software predicted that the Dicer1 deficiency-induced dysregulated cytokines in synoviocytes could possibly lead to the inflammatory disorders in the synovial tissue.
Moreover, DICER1 deficiency could also reduce apoptosis, while DICER1 overexpression was found to decrease the proliferation and enhance apoptosis.
In addition, DICER1 deficiency could lower the expression of multiple RA-related miRNAs such as miR-155.
Meanwhile, DICER1 overexpression could rescue their low expression levels.
And then, gain or loss of miR-155 function could regulate the protein levels of MMP3 and MMP13.
These results indicated that DICER1 might play its role through regulating its downstream RA-related miRNAs.
Our data demonstrated that DICER1 deficiency could cause multiple proinflammatory events in human synoviocytes SW982.
This mechanism study might provide the possible target molecule to modify the inflammatory destruction and overproliferation in synoviocytes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jiang, Congshan& Xu, Jing& Zhu, Wenhua& Cai, Yongsong& Wang, Si& Guo, Yuanxu…[et al.]. 2019. Abnormal Expression of DICER1 Leads to Dysregulation of Inflammatory Effectors in Human Synoviocytes. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1193169
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jiang, Congshan…[et al.]. Abnormal Expression of DICER1 Leads to Dysregulation of Inflammatory Effectors in Human Synoviocytes. Mediators of Inflammation No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1193169
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jiang, Congshan& Xu, Jing& Zhu, Wenhua& Cai, Yongsong& Wang, Si& Guo, Yuanxu…[et al.]. Abnormal Expression of DICER1 Leads to Dysregulation of Inflammatory Effectors in Human Synoviocytes. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1193169
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1193169
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر