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Application of Cerebrospinal Fluid Host Protein Biosignatures in the Diagnosis of Tuberculous Meningitis in Children from a High Burden Setting
المؤلفون المشاركون
Walzl, Gerhard
Stanley, Kim
Manyelo, Charles M.
Solomons, Regan S.
Snyders, Candice I.
Manngo, Portia M.
Mutavhatsindi, Hygon
Kriel, Belinda
Chegou, Novel N.
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-16
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Background.
The diagnosis of tuberculous meningitis (TBM) especially in children is challenging.
New tests are urgently needed for the diagnosis of the disease, especially in resource-limited settings.
Methods.
We collected cerebrospinal fluid (CSF) samples from children presenting with symptoms requiring investigation for meningitis at a tertiary hospital in Cape Town, South Africa.
Children were later classified as TBM or no TBM using published case definitions.
Using a multiplex platform, we investigated the concentrations of biomarkers comprising a previously established 3-marker biosignature (VEGF, IL-13, and LL-37) and other potentially useful host biomarkers as diagnostic candidates for TBM.
Findings.
Out of 47 children, age, 3 months to 13 years, 23 were diagnosed with TBM and six (16%) were HIV-infected.
We validated the previously identified CSF biosignature (sensitivity of 95.7% (95% CI, 79.0-99.2%) and specificity of 37.5% (95% CI, 21.2-57.3%)).
However, substitution of IL-13 and LL-37 with IFN-γ and MPO, respectively, resulted in improved accuracy (area under the ROC curve AUC=0.97, 95% CI, 0.92-1.00, up to 91.3% (21/23) sensitivity and up to 100% (24/24) specificity).
An alternative four-marker biosignature (sICAM-1, MPO, CXCL8, and IFN-γ) also showed potential, with an AUC of 0.97.
Conclusion.
We validated a previously identified CSF biosignature and showed that refinement of this biosignature by incorporation of other biomarkers diagnosed TBM with high accuracy.
Incorporation of these biomarkers into a point-of-care or bedside diagnostic test platform may result in the improved management of TBM in children.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Manyelo, Charles M.& Solomons, Regan S.& Snyders, Candice I.& Manngo, Portia M.& Mutavhatsindi, Hygon& Kriel, Belinda…[et al.]. 2019. Application of Cerebrospinal Fluid Host Protein Biosignatures in the Diagnosis of Tuberculous Meningitis in Children from a High Burden Setting. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1193290
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Manyelo, Charles M.…[et al.]. Application of Cerebrospinal Fluid Host Protein Biosignatures in the Diagnosis of Tuberculous Meningitis in Children from a High Burden Setting. Mediators of Inflammation No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1193290
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Manyelo, Charles M.& Solomons, Regan S.& Snyders, Candice I.& Manngo, Portia M.& Mutavhatsindi, Hygon& Kriel, Belinda…[et al.]. Application of Cerebrospinal Fluid Host Protein Biosignatures in the Diagnosis of Tuberculous Meningitis in Children from a High Burden Setting. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1193290
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1193290
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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