Pregnancy-Associated Plasma Protein A Induces Inflammatory Cytokine Expression by Activating IGF-IPI3KAkt Pathways
المؤلفون المشاركون
Li, Weiping
Li, Hongwei
Zhou, Li
Wang, Zijian
Hua, Bing
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-09-10
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Pregnancy-associated plasma protein A (PAPP-A) was previously reported to be an inflammatory biomarker and a prognostic marker of acute coronary syndrome (ACS) and involved in the process of atherosclerosis and plaque rupture.
However, the role of PAPP-A in inflammation is poorly understood.
In this study, we aimed to investigate the role of PAPP-A in macrophage activation and inflammatory cytokine production.
RAW264.7 macrophages were treated with or without PAPP-A.
Reverse-transcriptase quantitative real-time PCR (RT-qPCR) and Western blot were performed to detect gene and protein expressions.
The concentration of monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in culture supernatants was determined by ELISA.
Results showed that PAPP-A significantly stimulated the expression of MCP-1, TNF-α, and IL-6 at both transcriptional and translational levels in a dose-dependent and time-dependent manner.
The secretion of these inflammatory cytokines by macrophages was also increased after PAPP-A treatment.
Moreover, PAPP-A activated the IGF-I/PI3K/Akt signaling pathway in macrophages.
The PAPP-A-mediated upregulation of MCP-1, TNF-α, and IL-6 mRNA and protein levels were strongly inhibited by PI3K inhibitors or IGF-IR siRNA, indicating that the upregulation of MCP-1, TNF-α, and IL-6 could involve the IGF-I/PI3K/Akt pathway.
Together, this study demonstrates that PAPP-A activates the macrophage signaling pathway (IGF-I/PI3K/Akt), which drives the expression and production of inflammatory cytokines known to contribute to the initiation and progression of ACS.
These findings indicate that PAPP-A may play a proinflammatory role in the pathophysiology of ACS and serve as a potential therapeutic target.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Weiping& Li, Hongwei& Zhou, Li& Wang, Zijian& Hua, Bing. 2019. Pregnancy-Associated Plasma Protein A Induces Inflammatory Cytokine Expression by Activating IGF-IPI3KAkt Pathways. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1193478
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Weiping…[et al.]. Pregnancy-Associated Plasma Protein A Induces Inflammatory Cytokine Expression by Activating IGF-IPI3KAkt Pathways. Mediators of Inflammation No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1193478
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Weiping& Li, Hongwei& Zhou, Li& Wang, Zijian& Hua, Bing. Pregnancy-Associated Plasma Protein A Induces Inflammatory Cytokine Expression by Activating IGF-IPI3KAkt Pathways. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1193478
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1193478
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر