A Fast and Simple Qualitative Method for Screening Oleaginous Yeasts on Agar
المؤلفون المشاركون
Niehus, Xochitl
Casas-Godoy, Leticia
Vargas-Sánchez, Marcos
Sandoval, Georgina
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-07-29
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Finding new oleaginous yeasts is of great interest due to their many important applications.
Currently available screening procedures are time-consuming, and most of these require liquid cultures.
In this work, a new, fast, economical, and simple qualitative method for screening oleaginous yeasts was developed.
The fluorescent dye, Rhodamine B, was selected because its fluorescence is directly correlated to lipid content, and no additional steps or special equipment are needed.
This method only requires growing the yeasts on dyed agar plates.
Under visible light, it is easy to observe that nonpigmented oleaginous yeasts become colored, whereas non-oleaginous yeasts remain uncolored.
The developed method is also useful for improving medium composition in specific applications.
Moreover, it was also adapted to use alternative carbon sources, such as lignocellulosic materials and glycerol.
The developed method was applied to screen 124 recently isolated nonpigmented yeasts on three different carbon sources, namely, glucose, glycerol, and agave bagasse hydrolysate.
Five strains were selected as good lipid producers on all tested carbon sources and accumulated over 48% lipids.
Furthermore, the assay was adapted to screen reddish-pigmented yeasts.
Considering all the above, the developed method has a wide range of applications in the field of microbial oils.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Niehus, Xochitl& Casas-Godoy, Leticia& Vargas-Sánchez, Marcos& Sandoval, Georgina. 2018. A Fast and Simple Qualitative Method for Screening Oleaginous Yeasts on Agar. Journal of Lipids،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1193510
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Niehus, Xochitl…[et al.]. A Fast and Simple Qualitative Method for Screening Oleaginous Yeasts on Agar. Journal of Lipids No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1193510
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Niehus, Xochitl& Casas-Godoy, Leticia& Vargas-Sánchez, Marcos& Sandoval, Georgina. A Fast and Simple Qualitative Method for Screening Oleaginous Yeasts on Agar. Journal of Lipids. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1193510
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1193510
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر