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Apple Flavonoids Suppress Carcinogen-Induced DNA Damage in Normal Human Bronchial Epithelial Cells
المؤلفون المشاركون
George, Vazhappilly Cijo
Rupasinghe, H. P. Vasantha
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-06-18
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Scope.
Human neoplastic transformation due to DNA damage poses an increasing global healthcare concern.
Maintaining genomic integrity is crucial for avoiding tumor initiation and progression.
The present study aimed to investigate the efficacy of an apple flavonoid fraction (AF4) against various carcinogen-induced toxicity in normal human bronchial epithelial cells and its mechanism of DNA damage response and repair processes.
Methods and Results.
AF4-pretreated cells were exposed to nicotine-derived nitrosamine ketones (NNK), NNK acetate (NNK-Ae), methotrexate (MTX), and cisplatin to validate cytotoxicity, total reactive oxygen species, intracellular antioxidants, DNA fragmentation, and DNA tail damage.
Furthermore, phosphorylated histone (γ-H2AX) and proteins involved in DNA damage (ATM/ATR, Chk1, Chk2, and p53) and repair (DNA-PKcs and Ku80) mechanisms were evaluated by immunofluorescence and western blotting, respectively.
The results revealed that AF4-pretreated cells showed lower cytotoxicity, total ROS generation, and DNA fragmentation along with consequent inhibition of DNA tail moment.
An increased level of γ-H2AX and DNA damage proteins was observed in carcinogen-treated cells and that was significantly (p≤0.05) inhibited in AF4-pretreated cells, in an ATR-dependent manner.
AF4 pretreatment also facilitated the phosphorylation of DNA-PKcs and thus initiation of repair mechanisms.
Conclusion.
Apple flavonoids can protect in vitro oxidative DNA damage and facilitate repair mechanisms.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
George, Vazhappilly Cijo& Rupasinghe, H. P. Vasantha. 2017. Apple Flavonoids Suppress Carcinogen-Induced DNA Damage in Normal Human Bronchial Epithelial Cells. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1193942
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
George, Vazhappilly Cijo& Rupasinghe, H. P. Vasantha. Apple Flavonoids Suppress Carcinogen-Induced DNA Damage in Normal Human Bronchial Epithelial Cells. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1193942
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
George, Vazhappilly Cijo& Rupasinghe, H. P. Vasantha. Apple Flavonoids Suppress Carcinogen-Induced DNA Damage in Normal Human Bronchial Epithelial Cells. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1193942
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1193942
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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