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Moderate Autophagy Inhibits Vascular Smooth Muscle Cell Senescence to Stabilize Progressed Atherosclerotic Plaque via the mTORC1ULK1ATG13 Signal Pathway
المؤلفون المشاركون
Chen, Jiangwei
Cao, Feng
Qiao, Hongyu
Zhang, Ran
Luo, Zhenli
Xu, Wenhuan
Ma, Sai
Gao, Lei
Yang, Bo
Qiu, Ya
Zhang, Ming
Tao, Bo
Wang, Yabin
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-06-21
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
In order to investigate the effects of autophagy induced by rapamycin in the development of atherosclerosis plaque we established murine atherosclerosis model which was induced in ApoE−/− mice by high fat and cholesterol diet (HFD) for 16 weeks.
Rapamycin and 3-Methyladenine (MA) were used as autophagy inducer and inhibitor respectively.
The plaque areas in aortic artery were detected with HE and Oil Red O staining.
Immunohistochemical staining were applied to investigate content of plaque respectively.
In contrast to control and 3-MA groups, rapamycin could inhibit atherosclerosis progression.
Rapamycin was able to increase collagen content and a-SMA distribution relatively, as well as decrease necrotic core area.
Then we used MOVAS and culture with ox-LDL for 72 h to induce smooth muscle-derived foam cell model in vitro.
Rapamycin and 3-MA were cultured together respectively.
Flow cytometry assay and SA-β-Gal staining experiments were performed to detect survival and senescence of VSMCs.
Western blot analysis were utilized to analyze the levels of protein expression.
We found that rapamycin could promote ox-LDL-induced VSMCs autophagy survival and alleviate cellular senescence, in comparison to control and 3-MA groups.
Western blot analysis showed that rapamycin could upregulate ULK1, ATG13 and downregulate mTORC1 and p53 protein expression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Luo, Zhenli& Xu, Wenhuan& Ma, Sai& Qiao, Hongyu& Gao, Lei& Zhang, Ran…[et al.]. 2017. Moderate Autophagy Inhibits Vascular Smooth Muscle Cell Senescence to Stabilize Progressed Atherosclerotic Plaque via the mTORC1ULK1ATG13 Signal Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1194200
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Luo, Zhenli…[et al.]. Moderate Autophagy Inhibits Vascular Smooth Muscle Cell Senescence to Stabilize Progressed Atherosclerotic Plaque via the mTORC1ULK1ATG13 Signal Pathway. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1194200
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Luo, Zhenli& Xu, Wenhuan& Ma, Sai& Qiao, Hongyu& Gao, Lei& Zhang, Ran…[et al.]. Moderate Autophagy Inhibits Vascular Smooth Muscle Cell Senescence to Stabilize Progressed Atherosclerotic Plaque via the mTORC1ULK1ATG13 Signal Pathway. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1194200
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1194200
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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