Spermidine Oxidation-Mediated Degeneration of Retinal Pigment Epithelium in Rats
المؤلفون المشاركون
Ohashi, Koji
Kageyama, Masaaki
Shinomiya, Katsuhiko
Fujita-Koyama, Yukie
Hirai, Shin-ichiro
Katsuta, Osamu
Nakamura, Masatsugu
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-03-07
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Retinal pigment epithelium (RPE) degeneration is a crucial event in dry age-related macular degeneration and gyrate atrophy.
The polyamine spermidine has been shown to induce RPE cell death in vitro.
The present study aimed to establish a novel in vivo model of spermidine-induced RPE degeneration and to determine whether spermidine-induced RPE cell death involves oxidative mechanisms.
In this study, spermidine caused ARPE-19 cell death in a concentration-dependent manner.
This effect was prevented by removal of serum from the culture medium or treatment with amine oxidase inhibitors, N-acetylcysteine (NAC), or aldehyde dehydrogenase (ALDH).
Intravitreal injection of spermidine into rats significantly increased the permeability of the blood-retinal barrier and decreased the amplitudes of scotopic electroretinogram a- and b-waves.
Histological analysis revealed that spermidine induced vacuolation, atrophy, and dropout of RPE cells, leading to the disruption of photoreceptor outer segments.
Simultaneous intravitreal administration of NAC and ALDH with spermidine prominently inhibited the functional and morphological changes induced by spermidine.
In conclusion, this study demonstrated that the intravitreal administration of spermidine induced RPE cell dysfunction and death followed by photoreceptor degeneration in rats.
These effects of spermidine are thought to be mediated by oxidative stress and a toxic aldehyde generated during spermidine oxidation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ohashi, Koji& Kageyama, Masaaki& Shinomiya, Katsuhiko& Fujita-Koyama, Yukie& Hirai, Shin-ichiro& Katsuta, Osamu…[et al.]. 2017. Spermidine Oxidation-Mediated Degeneration of Retinal Pigment Epithelium in Rats. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1194576
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ohashi, Koji…[et al.]. Spermidine Oxidation-Mediated Degeneration of Retinal Pigment Epithelium in Rats. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1194576
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ohashi, Koji& Kageyama, Masaaki& Shinomiya, Katsuhiko& Fujita-Koyama, Yukie& Hirai, Shin-ichiro& Katsuta, Osamu…[et al.]. Spermidine Oxidation-Mediated Degeneration of Retinal Pigment Epithelium in Rats. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1194576
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1194576
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر