Zinc Protects Oxidative Stress-Induced RPE Death by Reducing Mitochondrial Damage and Preventing Lysosome Rupture
المؤلفون المشاركون
Xu, H.
Chen, Mei
Rajapakse, Dinusha
Curtis, Tim
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-14
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Zinc deficiency is known to increase the risk of the development of age-related macular degeneration (AMD), although the underlying mechanism remains poorly defined.
In this study, we investigated the effect of zinc on retinal pigment epithelium (RPE) survival and function under oxidative conditions.
Zinc level was 5.4 μM in normal culture conditions (DMEM/F12 with 10% FCS) and 1.5 μM in serum-free medium (DMEM/F12).
Under serum-free culture conditions, the treatment of RPE cells with oxidized photoreceptor outer segment (oxPOS) significantly increased intracellular ROS production, reduced ATP production, and promoted RPE death compared to oxPOS-treated RPE under normal culture condition.
Serum deprivation also reduced RPE phagocytosis of oxPOS and exacerbated oxidative insult-induced cathepsin B release from lysosome, an indicator of lysosome rupture.
The addition of zinc in the serum-free culture system dose dependently reduced ROS production, recovered ATP production, and reduced oxidative stress- (oxPOS- or 4-HNE) induced cell death.
Zinc supplementation also reduced oxidative stress-mediated cathepsin B release in RPE cells.
Our results suggest that zinc deficiency sensitizes RPE cells to oxidative damage, and zinc supplementation protects RPE cells from oxidative stress-induced death by improving mitochondrial function and preventing lysosome rupture.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rajapakse, Dinusha& Curtis, Tim& Chen, Mei& Xu, H.. 2017. Zinc Protects Oxidative Stress-Induced RPE Death by Reducing Mitochondrial Damage and Preventing Lysosome Rupture. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1195352
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rajapakse, Dinusha…[et al.]. Zinc Protects Oxidative Stress-Induced RPE Death by Reducing Mitochondrial Damage and Preventing Lysosome Rupture. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1195352
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rajapakse, Dinusha& Curtis, Tim& Chen, Mei& Xu, H.. Zinc Protects Oxidative Stress-Induced RPE Death by Reducing Mitochondrial Damage and Preventing Lysosome Rupture. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1195352
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1195352
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر