Xylopine Induces Oxidative Stress and Causes G2M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells
المؤلفون المشاركون
Soares, Milena Botelho
Costa, Emmanoel V.
Bezerra, Daniel Pereira
Santos, Luciano de Souza
Silva, Valdenizia Rodrigues
Menezes, Leociley Rocha Alencar
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-12-06
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Xylopine is an aporphine alkaloid that has cytotoxic activity to cancer cells.
In this study, the underlying mechanism of xylopine cytotoxicity was assessed in human colon carcinoma HCT116 cells.
Xylopine displayed potent cytotoxicity in different cancer cell lines in monolayer cultures and in a 3D model of cancer multicellular spheroids formed from HCT116 cells.
Typical morphology of apoptosis, cell cycle arrest in the G2/M phase, increased internucleosomal DNA fragmentation, loss of the mitochondrial transmembrane potential, and increased phosphatidylserine externalization and caspase-3 activation were observed in xylopine-treated HCT116 cells.
Moreover, pretreatment with a caspase-3 inhibitor (Z-DEVD-FMK), but not with a p53 inhibitor (cyclic pifithrin-α), reduced xylopine-induced apoptosis, indicating induction of caspase-mediated apoptosis by the p53-independent pathway.
Treatment with xylopine also caused an increase in the production of reactive oxygen/nitrogen species (ROS/RNS), including hydrogen peroxide and nitric oxide, but not superoxide anion, and reduced glutathione levels were decreased in xylopine-treated HCT116 cells.
Application of the antioxidant N-acetylcysteine reduced the ROS levels and xylopine-induced apoptosis, indicating activation of ROS-mediated apoptosis pathway.
In conclusion, xylopine has potent cytotoxicity to different cancer cell lines and is able to induce oxidative stress and G2/M phase arrest, triggering caspase-mediated apoptosis by the p53-independent pathway in HCT116 cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Santos, Luciano de Souza& Silva, Valdenizia Rodrigues& Menezes, Leociley Rocha Alencar& Soares, Milena Botelho& Costa, Emmanoel V.& Bezerra, Daniel Pereira. 2017. Xylopine Induces Oxidative Stress and Causes G2M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1195467
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Santos, Luciano de Souza…[et al.]. Xylopine Induces Oxidative Stress and Causes G2M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1195467
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Santos, Luciano de Souza& Silva, Valdenizia Rodrigues& Menezes, Leociley Rocha Alencar& Soares, Milena Botelho& Costa, Emmanoel V.& Bezerra, Daniel Pereira. Xylopine Induces Oxidative Stress and Causes G2M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1195467
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1195467
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر