REGγ Contributes to Regulation of Hemoglobin and Hemoglobin δ Subunit
المؤلفون المشاركون
Fu, Junjiang
Huang, Wenxiang
Li, Lei
Zuo, Qiuhong
Cheng, Shanshan
Bhatti, Muhammad Zeeshan
Xue, Yanyan
Zhang, Yuanyuan
Zhang, Bianhong
Wu, Lin
Chen, Jiwu
Li, Xiaotao
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-07-16
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Hemoglobin (Hb) is a family of proteins in red blood cells responsible for oxygen transport and vulnerable for oxidative damage.
Hemoglobin δ subunit (HBD), a member of Hb family, is normally expressed by cells of erythroid lineage.
Expression of Hb genes has been previously reported in nonerythroid and hematopoietic stem cells.
Here, we report that Hb and HBD can be degraded via REGγ proteasome in hemopoietic tissues and nonerythroid cells.
For this purpose, bone marrow, liver, and spleen hemopoietic tissues from REGγ+/+ and REGγ−/− mice and stable REGγ knockdown cells were evaluated for the degradation of Hb and HBD via REGγ.
Western blot and immunohistochemical analyses exhibited downregulation of Hb in REGγ wild-type mouse tissues.
This was validated by dynamic analysis following blockade of de novo synthesis of proteins with CHX.
Degradation of HBD only occurred in REGγ WT cells but not in REGγN151Y, a dominant-negative REGγ mutant cell.
Notably, downregulation of HBD was found in HeLa shN cells with stimulation of phenylhydrazine, an oxidation inducer, suggesting that the REGγ proteasome may target oxidatively damaged Hbs.
In conclusion, our findings provide important implications for the degradation of Hb and HBD in hemopoietic tissues and nonerythroid cells via the REGγ proteasome.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zuo, Qiuhong& Cheng, Shanshan& Huang, Wenxiang& Bhatti, Muhammad Zeeshan& Xue, Yanyan& Zhang, Yuanyuan…[et al.]. 2017. REGγ Contributes to Regulation of Hemoglobin and Hemoglobin δ Subunit. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1195575
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zuo, Qiuhong…[et al.]. REGγ Contributes to Regulation of Hemoglobin and Hemoglobin δ Subunit. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1195575
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zuo, Qiuhong& Cheng, Shanshan& Huang, Wenxiang& Bhatti, Muhammad Zeeshan& Xue, Yanyan& Zhang, Yuanyuan…[et al.]. REGγ Contributes to Regulation of Hemoglobin and Hemoglobin δ Subunit. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1195575
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1195575
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر