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Modulation of Glutathione Hemostasis by Inhibition of 1215-Lipoxygenase Prevents ROS-Mediated Cell Death after Hepatic Ischemia and Reperfusion
المؤلفون المشاركون
Guba, Markus
Holdt, Lesca
Khandoga, Andrej
Steib, Christian J.
Angele, M.
Drefs, Moritz
Thomas, Michael N.
Conrad, Marcus
Rentsch, Markus
Werner, Jens
Andrassy, Joachim
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-07-24
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Background.
Reactive oxygen species- (ROS-) mediated ischemia-reperfusion injury (IRI) detrimentally impacts liver transplantation and resection.
12/15-Lipoxygenase (12/15-LOX), an antagonistic protein of the glutathione peroxidase 4 (GPX4) signaling cascade, was proven to mediate cell death in postischemic cerebral and myocardial tissue.
The aim of this study was to investigate the impact of 12/15-LOX inhibition on hepatic IRI.
Methods.
Livers of C57BL/6 mice were exposed to 60 minutes of partial warm ischemia and 90 minutes of reperfusion after previous Baicalein administration, an inhibitor of 12/15-LOX.
Tissue samples were analyzed by TUNEL assay, Western blot, and spectral photometry.
Results.
TUNEL labeling showed a significant reduction of hepatic cell death following baicalein pretreatment.
Western Blot analysis revealed a significant downregulation of Jun-amino-terminal-kinase (JNK), caspase-3, and poly-ADP-ribose-polymerase (PARP), besides considerably lowered p44/42-MAP-kinase (ERK1/2) expression after Baicalein administration.
A significant elevation of glutathione oxidation was measured in Baicalein pretreated livers.
Conclusion.
Our data show that inhibition of 12/15-lipoxygenase causes significant cell death reduction after hepatic ischemia and reperfusion by enhancing glutathione metabolism.
We conclude that GPX4-dependent cell death signaling cascade might play a major role in development of hepatic IRI, in which the investigated proteins JNK, caspase-3, ERK1/2, and PARP might contribute to tissue damage.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Drefs, Moritz& Thomas, Michael N.& Guba, Markus& Angele, M.& Werner, Jens& Conrad, Marcus…[et al.]. 2017. Modulation of Glutathione Hemostasis by Inhibition of 1215-Lipoxygenase Prevents ROS-Mediated Cell Death after Hepatic Ischemia and Reperfusion. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1195995
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Drefs, Moritz…[et al.]. Modulation of Glutathione Hemostasis by Inhibition of 1215-Lipoxygenase Prevents ROS-Mediated Cell Death after Hepatic Ischemia and Reperfusion. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1195995
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Drefs, Moritz& Thomas, Michael N.& Guba, Markus& Angele, M.& Werner, Jens& Conrad, Marcus…[et al.]. Modulation of Glutathione Hemostasis by Inhibition of 1215-Lipoxygenase Prevents ROS-Mediated Cell Death after Hepatic Ischemia and Reperfusion. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1195995
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1195995
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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