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Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering
المؤلفون المشاركون
Huh, Man-Il
Kim, Hong Kyun
Lee, Kyoung-Pil
Kim, Jeongho
Yi, Soojin
Park, Byeong-Ung
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-01
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Purpose.
To establish an optimized and standardized protocol for the development of optimal scaffold for bioengineering corneal substitutes, we used femtosecond laser to process human corneal tissue into stromal lenticules and studied to find the most efficient decellularization method among various reagents with different tonicities.
Methods.
The decellularization efficacy of several agents (0.1%, 0.25%, and 0.5% of Triton X-100, SDS, and trypsin-EDTA (TE), resp.) with different tonicities was evaluated.
Of all protocols, the decellularization methods, which efficiently removed nuclear materials examined as detected by immunofluorescent staining, were quantitatively tested for sample DNA and glycosaminoglycan (GAG) contents, recellularization efficacy, and biocompatibilities.
Results.
0.5% SDS in hypertonic and isotonic buffer, 0.25% TE in hypotonic buffer, and 0.5% TE in all tonicities completely decellularized the corneal lenticules.
Of the protocols, decellularization with hypotonic 0.25 and 0.5% TE showed the lowest DNA contents, while the GAG content was the highest.
Furthermore, the recellularization efficacy of the hypotonic TE method was better than that of the SDS-based method.
Hypotonic TE-treated decellularized corneal lenticules (DCLs) were sufficiently transparent and biocompatible.
Conclusion.
We generated an ideal protocol for DCLs using a novel method.
Furthermore, it is possible to create a scaffold using a bioengineered corneal substitute.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Huh, Man-Il& Lee, Kyoung-Pil& Kim, Jeongho& Yi, Soojin& Park, Byeong-Ung& Kim, Hong Kyun. 2018. Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering. Journal of Ophthalmology،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1196333
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Huh, Man-Il…[et al.]. Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering. Journal of Ophthalmology No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1196333
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Huh, Man-Il& Lee, Kyoung-Pil& Kim, Jeongho& Yi, Soojin& Park, Byeong-Ung& Kim, Hong Kyun. Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering. Journal of Ophthalmology. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1196333
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1196333
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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