Ginkgolide B Suppresses TLR4-Mediated Inflammatory Response by Inhibiting the Phosphorylation of JAK2STAT3 and p38 MAPK in High Glucose-Treated HUVECs
المؤلفون المشاركون
Sun, Wenjia
Zhao, Yanyang
Chen, Beidong
Qi, Ruomei
Chen, Kun
Jiang, Yun
Sun, Jie
Gong, Huan
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-07-12
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Aim.
Ginkgolide B is a Ginkgo biloba leaf extract that has been identified as a natural platelet-activating factor receptor (PAFR) antagonist.
We investigated the effect of ginkgolide B on high glucose-induced TLR4 activation in human umbilical vein endothelial cells (HUVECs).
Methods.
Protein expression was analyzed by immunoblotting.
Small-interfering RNA (siRNA) was used to knock down PAFR and TLR4 expression.
Results.
Ginkgolide B suppressed the expression of TLR4 and MyD88 that was induced by high glucose.
Ginkgolide B also reduced the levels of platelet endothelial cell adhesion molecule-1, interleukin-6, and monocyte chemotactic protein 1.
Further, we examined the association between PAFR and TLR4 by coimmunoprecipitation.
The result showed that high glucose treatment caused the binding of PAFR and TLR4, whereas ginkgolide B abolished this binding.
The functional analysis indicated that PAFR siRNA treatment reduced TLR4 expression, and TLR4 siRNA treatment decreased PAFR expression in high glucose-treated HUVECs, further supporting the coimmunoprecipitation data.
Ginkgolide B inhibited the phosphorylation of Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) and p38 mitogen-activated protein kinase (MAPK).
Conclusion.
Ginkgolide B exerted protective effects by inhibiting the TLR4-mediated inflammatory response in high glucose-treated endothelial cells.
The mechanism of action of ginkgolide B might be associated with inhibition of the JAK2/STAT3 and p38 MAPK phosphorylation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Kun& Sun, Wenjia& Jiang, Yun& Chen, Beidong& Zhao, Yanyang& Sun, Jie…[et al.]. 2017. Ginkgolide B Suppresses TLR4-Mediated Inflammatory Response by Inhibiting the Phosphorylation of JAK2STAT3 and p38 MAPK in High Glucose-Treated HUVECs. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1196502
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Kun…[et al.]. Ginkgolide B Suppresses TLR4-Mediated Inflammatory Response by Inhibiting the Phosphorylation of JAK2STAT3 and p38 MAPK in High Glucose-Treated HUVECs. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1196502
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Kun& Sun, Wenjia& Jiang, Yun& Chen, Beidong& Zhao, Yanyang& Sun, Jie…[et al.]. Ginkgolide B Suppresses TLR4-Mediated Inflammatory Response by Inhibiting the Phosphorylation of JAK2STAT3 and p38 MAPK in High Glucose-Treated HUVECs. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1196502
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1196502
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر