Cyanin Chloride Inhibits Hyperbaric Pressure-Induced Decrease of Intracellular Glutamate-Aspartate Transporter in Rat Retinal Müller Cells
المؤلفون المشاركون
Ke, Min
Chen, Xiaomin
Wang, Yue
Han, Fangfang
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-10-31
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Purpose.
Glaucoma is the leading cause of irreversible blindness throughout the world.
The pathogenesis of glaucoma is complex, and neuroprotection is a crucial aspect of therapy.
High concentrations of extracellular glutamate are toxic to the optic nerve.
The glutamate-aspartate transporter (GLAST) in retinal Müller cells is involved in the development of glaucoma.
Anthocyanin has been reported to protect retinal neurons.
We hypothesize that cyanin chloride, a type of anthocyanin, can inhibit hyperbaric pressure-induced GLAST decreases in cultured rat retinal Müller cells and may serve as a potential neuroprotective agent in glaucoma treatment.
Materials and Methods.
Sprague Dawley rat Müller cells were cultured in a hyperbaric pressure device at 60 mmHg additional pressure and treated with cyanin chloride (10 μmol/L, 30 μmol/L, or 50 μmol/L) or vehicle for 2 hours.
Cell survival rates (SRs) were evaluated by an MTT assay.
GLAST mRNA and protein expression were determined by western blot and RT-PCR analyses, respectively.
Results.
Cell SR was significantly decreased in the 60 mmHg additional hyperbaric pressure group compared to the control group (P<0.01).
Cyanin chloride treatment significantly improved SR under 60 mmHg additional pressure (P<0.01).
GLAST mRNA and protein expression levels in Müller cells were significantly reduced in the 60 mmHg hyperbaric pressure group compared to the control group (P<0.01), but cyanin chloride significantly inhibited hyperbaric pressure-induced decreases in GLAST expression (P<0.01).
Conclusion.
Our results support our hypothesis and demonstrate that cyanin chloride can protect rat retinal Müller cells from hyperbaric pressure-induced decreases of GLAST.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Xiaomin& Wang, Yue& Han, Fangfang& Ke, Min. 2018. Cyanin Chloride Inhibits Hyperbaric Pressure-Induced Decrease of Intracellular Glutamate-Aspartate Transporter in Rat Retinal Müller Cells. Journal of Ophthalmology،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1196761
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Xiaomin…[et al.]. Cyanin Chloride Inhibits Hyperbaric Pressure-Induced Decrease of Intracellular Glutamate-Aspartate Transporter in Rat Retinal Müller Cells. Journal of Ophthalmology No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1196761
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Xiaomin& Wang, Yue& Han, Fangfang& Ke, Min. Cyanin Chloride Inhibits Hyperbaric Pressure-Induced Decrease of Intracellular Glutamate-Aspartate Transporter in Rat Retinal Müller Cells. Journal of Ophthalmology. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1196761
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1196761
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر