A Novel Model of Cancer-Induced Peripheral Neuropathy and the Role of TRPA1 in Pain Transduction
المؤلفون المشاركون
Elsadek, Bakheet E. M.
Maqboul, Ahmad
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-12-28
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Background.
Models of cancer-induced neuropathy are designed by injecting cancer cells near the peripheral nerves.
The interference of tissue-resident immune cells does not allow a direct contact with nerve fibres which affects the tumor microenvironment and the invasion process.
Methods.
Anaplastic tumor-1 (AT-1) cells were inoculated within the sciatic nerves (SNs) of male Copenhagen rats.
Lumbar dorsal root ganglia (DRGs) and the SNs were collected on days 3, 7, 14, and 21.
SN tissues were examined for morphological changes and DRG tissues for immunofluorescence, electrophoretic tendency, and mRNA quantification.
Hypersensitivities to cold, mechanical, and thermal stimuli were determined.
HC-030031, a selective TRPA1 antagonist, was used to treat cold allodynia.
Results.
Nociception thresholds were identified on day 6.
Immunofluorescent micrographs showed overexpression of TRPA1 on days 7 and 14 and of CGRP on day 14 until day 21.
Both TRPA1 and CGRP were coexpressed on the same cells.
Immunoblots exhibited an increase in TRPA1 expression on day 14.
TRPA1 mRNA underwent an increase on day 7 (normalized to 18S).
Injection of HC-030031 transiently reversed the cold allodynia.
Conclusion.
A novel and a promising model of cancer-induced neuropathy was established, and the role of TRPA1 and CGRP in pain transduction was examined.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Maqboul, Ahmad& Elsadek, Bakheet E. M.. 2017. A Novel Model of Cancer-Induced Peripheral Neuropathy and the Role of TRPA1 in Pain Transduction. Pain Research and Management،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1197379
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Maqboul, Ahmad& Elsadek, Bakheet E. M.. A Novel Model of Cancer-Induced Peripheral Neuropathy and the Role of TRPA1 in Pain Transduction. Pain Research and Management No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1197379
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Maqboul, Ahmad& Elsadek, Bakheet E. M.. A Novel Model of Cancer-Induced Peripheral Neuropathy and the Role of TRPA1 in Pain Transduction. Pain Research and Management. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1197379
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1197379
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر