Dermatopathology of Orf Virus (Malaysian Isolates)‎ in Mice Experimentally Inoculated at Different Sites with and without Dexamethasone Administration

المؤلفون المشاركون

Mohamed Mustapha, Noordin
Abba, Yusuf
Jesse, F. F. A.
Haron, A. W.
Lila, M. A. M.
Bala, Jamilu Abubakar
Balakrishnan, Krishnan Nair
Abdullah, Ashwaq Ahmed
Kimmy, Tay
Bin Mohamed, Ramlan
Bitrus, Asinamai Athliamai
Hambali, Idris Umar

المصدر

Journal of Pathogens

العدد

المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2018-08-01

دولة النشر

مصر

عدد الصفحات

12

التخصصات الرئيسية

الأمراض
الطب البشري

الملخص EN

Orf is a clinical manifestation of parapoxvirus infection often fatal in goats and sheep especially when they are under stress or influenced by unfavorable environment.

This study investigated the pathogenicity of two Orf virus isolates (ORFV UPM1/14 and UPM2/14) and host response in mouse model by using different inoculation sites with/without prior exposure to dexamethasone.

Treatments with dexamethasone served as an immunosuppressant that may mimic stress situation in affected animals.

Groups of five mice were given intradermal injection of 0.2 mL of tissue culture infective dose 50 (TCID50) of UPM1/14 (Group 1) and UPM2/14 (Group 2) at the dorsum (Group 1A; Group 2A), ear pinna (Group 1B; Group 2B), and labial commissure (Group 1C; Group 2C).

An inoculum 0.2 mL of UPM1/14 was administered to animals treated with dexamethasone (n=5; 5 mg/kg/day intraperitoneally) and nondexamethasone (n=5) groups at the dorsum, ear pinna, and labial commissure.

No significant difference (p>0.05) was observed in the mean lesion scores among the groups of different inoculation sites or between dexamethasone-treated and nontreated groups.

However, there was a significant difference (p<0.05) in the mean stratum thickness of affected skin following inoculation with UPM2/14 isolate at the ear pinna and labial commissure.

Histopathology examination revealed keratosis, acanthosis, and ballooning degeneration in the skin of affected mice.

Orf virus DNA was detected in the skin samples by targeting F1L and B2L virus-specific genes in polymerase chain reaction (PCR) assay.

Intradermal inoculation with UPM1/14 or UPM2/14 isolate produced a mild skin lesion in mice, and there was no significant difference in orf disease manifestation despite variation of inoculation sites.

Similarly, short-term dexamethasone administration gave no adverse effects on pathogenicity of orf virus isolates.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Bala, Jamilu Abubakar& Balakrishnan, Krishnan Nair& Abdullah, Ashwaq Ahmed& Kimmy, Tay& Abba, Yusuf& Bin Mohamed, Ramlan…[et al.]. 2018. Dermatopathology of Orf Virus (Malaysian Isolates) in Mice Experimentally Inoculated at Different Sites with and without Dexamethasone Administration. Journal of Pathogens،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1197536

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Bala, Jamilu Abubakar…[et al.]. Dermatopathology of Orf Virus (Malaysian Isolates) in Mice Experimentally Inoculated at Different Sites with and without Dexamethasone Administration. Journal of Pathogens No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1197536

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Bala, Jamilu Abubakar& Balakrishnan, Krishnan Nair& Abdullah, Ashwaq Ahmed& Kimmy, Tay& Abba, Yusuf& Bin Mohamed, Ramlan…[et al.]. Dermatopathology of Orf Virus (Malaysian Isolates) in Mice Experimentally Inoculated at Different Sites with and without Dexamethasone Administration. Journal of Pathogens. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1197536

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1197536