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Molecular Detection of Brucella spp. from Milk of Seronegative Cows from Some Selected Area in Bangladesh
المؤلفون المشاركون
Islam, Md. Sadequl
Islam, Md. Ariful
Khatun, Mst. Minara
Saha, Sukumar
Basir, Md. Samiul
Hasan, Md- Mahmodul
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-01-14
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Brucellosis is endemic in Bangladesh both in humans and in animals.
A number of reasons complicate the diagnosis, as bovine brucellosis can be diagnosed by various serological tests.
But the tests have a limitation; when the organism remains intracellular, the disease goes into chronic stage and the antibody titres may decline.
The present study was conducted for isolation and detection of Brucella spp.
by polymerase chain reaction (PCR) from seronegative cows.
A total of 360 dairy cows from three geographical regions were screened serologically by Rose Bengal Plate Test (RBPT) where 24 samples were serologically positive and the rest of the samples were serologically negative.
Among the 24 seropositive individuals, 11 were culture positive and 6 were culture positive from serologically negative dairy cows.
The overall seroprevalence of brucellosis in cattle was 6.6% and in disease condition a higher prevalence was recorded in abortion (28.07%) followed by infertility (13.33%).
To confirm the Brucella spp.
in seronegative dairy cattle, the isolates were extracted and PCR was conducted, which produced 905 bp amplicon size of 6 Brucella spp.
from milk sample.
So, for the detection or eradication of brucellosis, a bacteriological test and a PCR technique should be performed with the serological test of milk.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Islam, Md. Sadequl& Islam, Md. Ariful& Khatun, Mst. Minara& Saha, Sukumar& Basir, Md. Samiul& Hasan, Md- Mahmodul. 2018. Molecular Detection of Brucella spp. from Milk of Seronegative Cows from Some Selected Area in Bangladesh. Journal of Pathogens،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1197544
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Islam, Md. Sadequl…[et al.]. Molecular Detection of Brucella spp. from Milk of Seronegative Cows from Some Selected Area in Bangladesh. Journal of Pathogens No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1197544
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Islam, Md. Sadequl& Islam, Md. Ariful& Khatun, Mst. Minara& Saha, Sukumar& Basir, Md. Samiul& Hasan, Md- Mahmodul. Molecular Detection of Brucella spp. from Milk of Seronegative Cows from Some Selected Area in Bangladesh. Journal of Pathogens. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1197544
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1197544
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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