Resveratrol Induces Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Neuron-Like Cells
المؤلفون المشاركون
Li, Depei
Guo, Li
Wang, Liang
Wang, Li
Yun-peng, Shi
Zhou, Jing-jing
Zhao, Zongmao
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-04-20
دولة النشر
مصر
عدد الصفحات
7
الملخص EN
Objective.
Human umbilical cord mesenchymal stem cells (hUC-MSCs) potentially differentiate to various types of cells including neuron-like cells.
The natural polyphenol resveratrol benefits patients with many diseases including ischemic brain injury.
We hypothesize that resveratrol induces differentiation of hUC-MSCs into neuron-like cells.
Methods.
Flow cytometry was used to determine the surface antigens in different stage of hUC-MSCs (P2, P5, and P10).
Nestin, neuron-specific enolase (NSE), and glial fibrillary acidic protein (GFAP) were detected by immunocytochemistry, Western blotting, and real time RT-PCT.
The cultured hUC-MSCs were treated with resveratrol at different concentrations (0, 7.5, 15.0, and 30.0 mg/L).
Nestin, GFAP, and NSE protein and mRNA were measured at posttreatment time points of 2 h, 4 h, 6 h, 12 h, and 24 h.
Results.
Neuron-like cells were found in hUC-MSCs treated by resveratrol at concentrations of 15.0 and 30.0 mg/L, but not in hUC-MSCs treated with vehicle and 7.5 mg/L resveratrol.
Furthermore, immunocytochemical staining revealed that nestin and NSE immunoreactivities were positive in resveratrol-treated hUC-MSCs at concentrations of 15.0 and 30.0 mg/L.
Resveratrol treatment significantly increased nestin and NSE protein and mRNA levels 4 h after the treatment.
However, resveratrol treatment did not change GFAP immunoreactivities and protein and mRNA expression levels in cultured hUC-MSCs.
Conclusions.
Taken together, resveratrol treatment induces a differentiation of hUC-MSCs into neuron-like cells at relatively high concentrations.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Guo, Li& Wang, Liang& Wang, Li& Yun-peng, Shi& Zhou, Jing-jing& Zhao, Zongmao…[et al.]. 2017. Resveratrol Induces Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Neuron-Like Cells. Stem Cells International،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1200782
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Guo, Li…[et al.]. Resveratrol Induces Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Neuron-Like Cells. Stem Cells International No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1200782
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Guo, Li& Wang, Liang& Wang, Li& Yun-peng, Shi& Zhou, Jing-jing& Zhao, Zongmao…[et al.]. Resveratrol Induces Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Neuron-Like Cells. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1200782
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1200782
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر