Human Bone Marrow Mesenchymal StemStromal Cells Preserve Their Immunomodulatory and Chemotactic Properties When Expanded in a Human Plasma Derived Xeno-Free Medium

الملخص EN

Due to their immunomodulatory and chemotactic properties, hMSC are being explored to treat immune-related diseases.

For their use in human therapies, it is necessary to culture hMSC in xeno-free conditions.

In this study, the impact that a xeno-free medium based on a human plasma derivate has on these properties was analysed.

Bone marrow-derived hMSC preserved their immunosuppressive and immunostimulatory properties, as observed with in vitro assays with hMSC cocultured with mixed leukocyte reactions or with mitogen-stimulated leukocytes.

Moreover, hMSC expanded in xeno-free medium were recruited by macrophages in both migration and invasion assays, which indicates that the cells maintained their chemotactic properties.

These data suggest that xeno-free expanded hMSC preserved their immunomodulatory and chemotactic properties, indicating that the described xeno-free medium composition is a potential candidate to culture and expand hMSC for human cell therapies.