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The Impact of GFP Reporter Gene Transduction and Expression on Metabolomics of Placental Mesenchymal Stem Cells Determined by UHPLC-QTOF-MS
المؤلفون المشاركون
Cao, Hong-Cui
Dong, Xiaotian
Yu, Jiong
Wang, Dan
Liu, Jingqi
Pan, Qiaoling
Yang, Jinfeng
Wang, Nan
Chen, Deying
Shi, Xiaowei
Li, Liang
Li, Lanjuan
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-05
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Introduction.
Green fluorescent protein (GFP) is widely used as a reporter gene in regenerative medicine research to label and track stem cells.
Here, we examined whether expressing GFP gene may impact the metabolism of human placental mesenchymal stem cells (hPMSCs).
Methods.
The GFP gene was transduced into hPMSCs using lentiviral-based infection to establish GFP+hPMSCs.
A sensitive 13C/12C-dansyl labeling LC-MS method targeting the amine/phenol submetabolome was used for in-depth cell metabolome profiling.
Results.
A total of 1151 peak pairs or metabolites were detected from 12 LC-MS runs.
Principal component analysis and partial least squares discriminant analysis showed poor separation, and the volcano plots demonstrated that most of the metabolites were not significantly changed when hPMSCs were tagged with GFP.
Overall, 739 metabolites were positively or putatively identified.
Only 11 metabolites showed significant changes.
Metabolic pathway analyses indicated that three of the identified metabolites were involved in nine pathways.
However, these metabolites are unlikely to have a large impact on the metabolic pathways due to their nonessential roles and limited hits in pathway analysis.
Conclusion.
This study indicated that the expression of ectopic GFP reporter gene did not significantly alter the metabolomics pathways covered by the amine/phenol submetabolome.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Jinfeng& Wang, Nan& Chen, Deying& Yu, Jiong& Pan, Qiaoling& Wang, Dan…[et al.]. 2017. The Impact of GFP Reporter Gene Transduction and Expression on Metabolomics of Placental Mesenchymal Stem Cells Determined by UHPLC-QTOF-MS. Stem Cells International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1201125
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Jinfeng…[et al.]. The Impact of GFP Reporter Gene Transduction and Expression on Metabolomics of Placental Mesenchymal Stem Cells Determined by UHPLC-QTOF-MS. Stem Cells International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1201125
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Jinfeng& Wang, Nan& Chen, Deying& Yu, Jiong& Pan, Qiaoling& Wang, Dan…[et al.]. The Impact of GFP Reporter Gene Transduction and Expression on Metabolomics of Placental Mesenchymal Stem Cells Determined by UHPLC-QTOF-MS. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1201125
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1201125
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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