Endometritis and In Vitro PGE2 Challenge Modify Properties of Cattle Endometrial Mesenchymal Stem Cells and Their Transcriptomic Profile
المؤلفون المشاركون
Lara, Evelyn
Velásquez, Alejandra
Cabezas, Joel
Rivera, Nathaly
Pacha, Paulina
Rodríguez-Alvarez, Lleretny
Saravia, Fernando
Castro, Fidel Ovidio
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-10-29
دولة النشر
مصر
عدد الصفحات
16
الملخص EN
Mesenchymal stem cells (MSCs) were isolated and characterized from postpartum bovine endometrium of animals with subclinical (n=5) and clinical endometritis (n=3) and healthy puerperal females (n=5).
Cells isolated displayed mean morphological features of MSCs and underwent osteogenic, chondrogenic, and adipogenic differentiation after induction (healthy and subclinical).
Cells from cows with clinical endometritis did not undergo adipogenic differentiation.
All cells expressed mRNAs for selected MSC markers.
Endometrial MSCs were challenged in vitro with PGE2 at concentrations of 0, 1, 3, and 10 μM, and their global transcriptomic profile was studied.
Overall, 1127 genes were differentially expressed between unchallenged cells and cells treated with PGE2 at all concentrations (763 up- and 364 downregulated, fold change > 2, and P<0.05).
The pathways affected the most by the PGE2 challenge were immune response, angiogenesis, and cell proliferation.
In conclusion, we demonstrated that healthy puerperal bovine endometrium contains MSCs and that endometritis modifies and limits some functional characteristics of these cells, such as their ability to proceed to adipogenic differentiation.
Also, PGE2, an inflammatory mediator of endometritis, modifies the transcriptomic profile of endometrial MSCs.
A similar situation may occur during inflammation associated with endometritis, therefore affecting the main properties of endometrial MSCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lara, Evelyn& Velásquez, Alejandra& Cabezas, Joel& Rivera, Nathaly& Pacha, Paulina& Rodríguez-Alvarez, Lleretny…[et al.]. 2017. Endometritis and In Vitro PGE2 Challenge Modify Properties of Cattle Endometrial Mesenchymal Stem Cells and Their Transcriptomic Profile. Stem Cells International،Vol. 2017, no. 2017, pp.1-16.
https://search.emarefa.net/detail/BIM-1201552
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lara, Evelyn…[et al.]. Endometritis and In Vitro PGE2 Challenge Modify Properties of Cattle Endometrial Mesenchymal Stem Cells and Their Transcriptomic Profile. Stem Cells International No. 2017 (2017), pp.1-16.
https://search.emarefa.net/detail/BIM-1201552
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lara, Evelyn& Velásquez, Alejandra& Cabezas, Joel& Rivera, Nathaly& Pacha, Paulina& Rodríguez-Alvarez, Lleretny…[et al.]. Endometritis and In Vitro PGE2 Challenge Modify Properties of Cattle Endometrial Mesenchymal Stem Cells and Their Transcriptomic Profile. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-16.
https://search.emarefa.net/detail/BIM-1201552
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1201552
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر