Overexpression of Heme Oxygenase-1 in Mesenchymal Stem Cells Augments Their Protection on Retinal Cells In Vitro and Attenuates Retinal IschemiaReperfusion Injury In Vivo against Oxidative Stress

المؤلفون المشاركون

Zhou, Jin
Li, Li
Du, GaiPing
Wang, DaJiang
Jiang, Guomin
Jiang, Hua

المصدر

Stem Cells International

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-13، 13ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-02-01

دولة النشر

مصر

عدد الصفحات

13

الملخص EN

Retinal ischemia/reperfusion (I/R) injury, involving several ocular diseases, seriously threatens human ocular health, mainly treated by attenuating I/R-induced oxidative stress.

Currently, mesenchymal stem cells (MSCs) could restore I/R-injured retina through paracrine secretion.

Additionally, heme oxygenase-1 (HO-1) could ameliorate oxidative stress and thus retinal apoptosis, but the expression of HO-1 in MSC is limited.

Here, we hypothesized that overexpression of HO-1 in MSC (MSC-HO-1) may significantly improve their retina-protective potentials.

The overexpression of HO-1 in MSC was achieved by lentivirus transduction.

Then, MSC or MSC-HO-1 was cocultured with retinal ganglion cells (RGC-5) in H2O2-simulated oxidative condition and their protection on RGC-5 was systemically valuated in vitro.

Compared with MSC, MSC-HO-1 significantly attenuated H2O2-induced injury of RGC-5, including decrease in cellular ROS level and apoptosis, activation of antiapoptotic proteins p-Akt and Bcl-2, and blockage of proapoptotic proteins cleaved caspase 3 and Bax.

In retinal I/R rats model, compared with control MSC, MSC-HO-1-treated retina significantly retrieved its structural thickness, reduced cell apoptosis, markedly attenuated retinal oxidative stress level, and largely regained the activities of typical antioxidant enzymes, SOD and CAT.

Therefore, it could be concluded that overexpression of HO-1 provides a promising strategy to enhance the MSC-based therapy for I/R-related retinal injury.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Li, Li& Du, GaiPing& Wang, DaJiang& Zhou, Jin& Jiang, Guomin& Jiang, Hua. 2017. Overexpression of Heme Oxygenase-1 in Mesenchymal Stem Cells Augments Their Protection on Retinal Cells In Vitro and Attenuates Retinal IschemiaReperfusion Injury In Vivo against Oxidative Stress. Stem Cells International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1201704

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Li, Li…[et al.]. Overexpression of Heme Oxygenase-1 in Mesenchymal Stem Cells Augments Their Protection on Retinal Cells In Vitro and Attenuates Retinal IschemiaReperfusion Injury In Vivo against Oxidative Stress. Stem Cells International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1201704

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Li, Li& Du, GaiPing& Wang, DaJiang& Zhou, Jin& Jiang, Guomin& Jiang, Hua. Overexpression of Heme Oxygenase-1 in Mesenchymal Stem Cells Augments Their Protection on Retinal Cells In Vitro and Attenuates Retinal IschemiaReperfusion Injury In Vivo against Oxidative Stress. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1201704

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1201704