Effect of Hypoxia on the Differentiation and the Self-Renewal of Metanephrogenic Mesenchymal Stem Cells
المؤلفون المشاركون
Ding, Xiao-qiang
Liu, Shaopeng
Song, Nana
He, Jianqiang
Yu, Xiaofang
Guo, Jia
Jiao, Xiaoyan
Teng, Jie
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-01-17
دولة النشر
مصر
عدد الصفحات
16
الملخص EN
Hypoxia is an important and influential factor in development.
The embryonic kidney is exposed to a hypoxic environment throughout its development.
The Wnt/β-catenin pathway plays vital roles in the differentiation and self-renewal of metanephrogenic mesenchymal stem cells (MMSCs) from which the kidney is derived.
Thus, we hypothesized that hypoxia can regulate the differentiation and pluripotency of MMSCs through the Wnt/β-catenin pathway.
To test this hypothesis, MMSCs from rats at embryonic day 18.5 were cultured in normoxic (21% O2) and hypoxic (1% O2) conditions.
The effects of hypoxia on differentiation, stemness, proliferation, and apoptosis of cultured MMSCs and on the activity of the Wnt/β-catenin pathway were tested.
Our results revealed that the hypoxic condition increased the number of epithelial cells (E-cadherin+ or CK18+) as well the expression of markers of renal tubule epithelia cells (CDH6, Aqp1, and OPN), decreased the number and proliferation of stem cells (SIX-2+ or CITED1+), and induced apoptosis.
Additionally, hypoxia reduced the expression of Wnt4 as well as its downstream molecules β-catenin, LEF-1, and Axin2.
Activation of the Wnt/β-catenin pathway by LiCl or BIO modified the effects of hypoxia on the differentiation and self-renewal of MMSCs.
Thus, we concluded that hypoxia induces the differentiation and inhibits the self-renewal of MMSCs by inhibiting the Wnt/β-catenin pathway.
The observations further our understanding of the effects of hypoxia on kidney.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Shaopeng& Song, Nana& He, Jianqiang& Yu, Xiaofang& Guo, Jia& Jiao, Xiaoyan…[et al.]. 2017. Effect of Hypoxia on the Differentiation and the Self-Renewal of Metanephrogenic Mesenchymal Stem Cells. Stem Cells International،Vol. 2017, no. 2017, pp.1-16.
https://search.emarefa.net/detail/BIM-1202040
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Shaopeng…[et al.]. Effect of Hypoxia on the Differentiation and the Self-Renewal of Metanephrogenic Mesenchymal Stem Cells. Stem Cells International No. 2017 (2017), pp.1-16.
https://search.emarefa.net/detail/BIM-1202040
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Shaopeng& Song, Nana& He, Jianqiang& Yu, Xiaofang& Guo, Jia& Jiao, Xiaoyan…[et al.]. Effect of Hypoxia on the Differentiation and the Self-Renewal of Metanephrogenic Mesenchymal Stem Cells. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-16.
https://search.emarefa.net/detail/BIM-1202040
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1202040
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر