Establishment of Novel Limbus-Derived, Highly Proliferative ABCG2+ABCB5+ Limbal Epithelial Stem Cell Cultures
المؤلفون المشاركون
Maeng, Yong-Sun
Kim, Eung Kweon
Lee, Ga-Hyun
Lee, Boram
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-05
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Homeostasis and regeneration of corneal epithelia are sustained by limbal epithelial stem cells (LESCs); thus, an LESC deficiency is a major cause of blindness worldwide.
Despite the generally promising results of cultivated LESC transplantation, it has been limited by variations in long-term success rates, the use of xenogeneic and undefined culture components, and a scarcity of donor tissues.
In this study, we identified the culture conditions required to expand LESCs in vitro and established human limbus-derived highly proliferative ABCG2+/ABCB5+ double-positive LESCs.
These LESCs exhibited the LESC marker profile and differentiated into corneal epithelial cells.
In addition, cultured LESCs expressed high levels of the stem cell markers Sox2, Oct4, c-Myc, and Klf4, had high telomerase activity, and had stable, normal genomes.
These results suggest that our novel cultivation protocol affects the phenotype and differentiation capacity of LESCs.
From the limbus, which contains a heterogenous cell population, we have derived highly proliferative ABCG2+/ABCB5+ double-positive cells with the ability to differentiate into corneal epithelial cells.
This study opens a new avenue for investigation of the molecular mechanism of LESC maintenance and expansion in vitro and may impact the treatment of corneal disease, particularly corneal blindness due to an LESC deficiency.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kim, Eung Kweon& Lee, Ga-Hyun& Lee, Boram& Maeng, Yong-Sun. 2017. Establishment of Novel Limbus-Derived, Highly Proliferative ABCG2+ABCB5+ Limbal Epithelial Stem Cell Cultures. Stem Cells International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1202170
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kim, Eung Kweon…[et al.]. Establishment of Novel Limbus-Derived, Highly Proliferative ABCG2+ABCB5+ Limbal Epithelial Stem Cell Cultures. Stem Cells International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1202170
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kim, Eung Kweon& Lee, Ga-Hyun& Lee, Boram& Maeng, Yong-Sun. Establishment of Novel Limbus-Derived, Highly Proliferative ABCG2+ABCB5+ Limbal Epithelial Stem Cell Cultures. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1202170
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1202170
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر