Platelet Lysate: The Better Choice for Jaw Periosteal Cell Mineralization
المؤلفون المشاركون
Reinert, Siegmar
Alexander, Dorothea
Wanner, Yvonne
Umrath, Felix
Waidmann, Marc
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-12-17
دولة النشر
مصر
عدد الصفحات
10
الملخص EN
Previously, we demonstrated a high quality of minerals formed by serum-free cultured jaw periosteal cells (JPCs) by Raman spectroscopy but the mineralization extent was not satisfactory.
In the present study, we analyzed the proliferation and mineralization potential of human platelet lysate- (hPL-) cultured JPCs in comparison to that of FCS-cultured JPCs.
By cell impedance measurements, we detected significantly higher population doubling times of PL-cultured JPCs in comparison to FCS-cultured JPCs.
However, this result was not based on lower proliferation activities but on diminished cell sizes which JPCs develop under PL cultivation.
The measurements of the metabolic activities clearly showed significantly higher cell proliferation rates under PL culturing.
Equivalent levels of the mesenchymal cell markers CD29, CD45, CD73, CD90, and CD105 were detected, but there were significantly increased MSCA-1 levels under PL cultivation.
While JPCs only occasionally mineralize under FCS culture conditions, the mineralization potential was significantly stronger under PL cultivation.
Moreover, in 4 of 5 analyzed patient cells, the addition of dexamethasone was proved no longer necessary for strong mineralization of PL-cultured JPCs.
We conclude that in vitro cultivation of JPCs with platelet lysate is a suitable alternative to FCS culture conditions and a powerful tool for the development of high-quality TE constructs using jaw periosteal cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wanner, Yvonne& Umrath, Felix& Waidmann, Marc& Reinert, Siegmar& Alexander, Dorothea. 2017. Platelet Lysate: The Better Choice for Jaw Periosteal Cell Mineralization. Stem Cells International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1202240
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wanner, Yvonne…[et al.]. Platelet Lysate: The Better Choice for Jaw Periosteal Cell Mineralization. Stem Cells International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1202240
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wanner, Yvonne& Umrath, Felix& Waidmann, Marc& Reinert, Siegmar& Alexander, Dorothea. Platelet Lysate: The Better Choice for Jaw Periosteal Cell Mineralization. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1202240
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1202240
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر