Capacity of Human Dental Follicle Cells to Differentiate into Neural Cells In Vitro
المؤلفون المشاركون
Ito, Ko
Kuyama, Kayo
Ogura, Naomi
Kondoh, Toshirou
Kanao, Shingo
Takahashi, Kosuke
Suemitsu, Masaaki
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-02-05
دولة النشر
مصر
عدد الصفحات
10
الملخص EN
The dental follicle is an ectomesenchymal tissue surrounding the developing tooth germ.
Human dental follicle cells (hDFCs) have the capacity to commit to differentiation into multiple cell types.
Here we investigated the capacity of hDFCs to differentiate into neural cells and the efficiency of a two-step strategy involving floating neurosphere-like bodies for neural differentiation.
Undifferentiated hDFCs showed a spindle-like morphology and were positive for neural markers such as nestin, β-III-tubulin, and S100β.
The cellular morphology of several cells was neuronal-like including branched dendrite-like processes and neurites.
Next, hDFCs were used for neurosphere formation in serum-free medium containing basic fibroblast growth factor, epidermal growth factor, and B27 supplement.
The number of cells with neuronal-like morphology and that were strongly positive for neural markers increased with sphere formation.
Gene expression of neural markers also increased in hDFCs with sphere formation.
Next, gene expression of neural markers was examined in hDFCs during neuronal differentiation after sphere formation.
Expression of Musashi-1 and Musashi-2, MAP2, GFAP, MBP, and SOX10 was upregulated in hDFCs undergoing neuronal differentiation via neurospheres, whereas expression of nestin and β-III-tubulin was downregulated.
In conclusion, hDFCs may be another optimal source of neural/glial cells for cell-based therapies to treat neurological diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kanao, Shingo& Ogura, Naomi& Takahashi, Kosuke& Ito, Ko& Suemitsu, Masaaki& Kuyama, Kayo…[et al.]. 2017. Capacity of Human Dental Follicle Cells to Differentiate into Neural Cells In Vitro. Stem Cells International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1202258
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kanao, Shingo…[et al.]. Capacity of Human Dental Follicle Cells to Differentiate into Neural Cells In Vitro. Stem Cells International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1202258
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kanao, Shingo& Ogura, Naomi& Takahashi, Kosuke& Ito, Ko& Suemitsu, Masaaki& Kuyama, Kayo…[et al.]. Capacity of Human Dental Follicle Cells to Differentiate into Neural Cells In Vitro. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1202258
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1202258
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر