Neural Differentiation Is Inhibited through HIF1αβ-Catenin Signaling in Embryoid Bodies
المؤلفون المشاركون
Večeřa, Josef
Kudová, Jana
Kučera, Jan
Pacherník, J.
Kubala, Lukas
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-12-20
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Extensive research in the field of stem cells and developmental biology has revealed evidence of the role of hypoxia as an important factor regulating self-renewal and differentiation.
However, comprehensive information about the exact hypoxia-mediated regulatory mechanism of stem cell fate during early embryonic development is still missing.
Using a model of embryoid bodies (EBs) derived from murine embryonic stem cells (ESC), we here tried to encrypt the role of hypoxia-inducible factor 1α (HIF1α) in neural fate during spontaneous differentiation.
EBs derived from ESC with the ablated gene for HIF1α had abnormally increased neuronal characteristics during differentiation.
An increased neural phenotype in Hif1α−/− EBs was accompanied by the disruption of β-catenin signaling together with the increased cytoplasmic degradation of β-catenin.
The knock-in of Hif1α, as well as β-catenin ectopic overexpression in Hif1α−/− EBs, induced a reduction in neural markers to the levels observed in wild-type EBs.
Interestingly, direct interaction between HIF1α and β-catenin was demonstrated by immunoprecipitation analysis of the nuclear fraction of wild-type EBs.
Together, these results emphasize the regulatory role of HIF1α in β-catenin stabilization during spontaneous differentiation, which seems to be a crucial mechanism for the natural inhibition of premature neural differentiation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Večeřa, Josef& Kudová, Jana& Kučera, Jan& Kubala, Lukas& Pacherník, J.. 2017. Neural Differentiation Is Inhibited through HIF1αβ-Catenin Signaling in Embryoid Bodies. Stem Cells International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1202283
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Večeřa, Josef…[et al.]. Neural Differentiation Is Inhibited through HIF1αβ-Catenin Signaling in Embryoid Bodies. Stem Cells International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1202283
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Večeřa, Josef& Kudová, Jana& Kučera, Jan& Kubala, Lukas& Pacherník, J.. Neural Differentiation Is Inhibited through HIF1αβ-Catenin Signaling in Embryoid Bodies. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1202283
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1202283
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر