Age-Dependent Oxidative Stress Elevates Arginase 1 and Uncoupled Nitric Oxide Synthesis in Skeletal Muscle of Aged Mice
المؤلفون المشاركون
Caldwell, R. William
Fulzele, Sadanand
Hamrick, Mark W.
Pandya, Chirayu D.
Lee, Byung
Toque, Haroldo A.
Mendhe, Bharati
Bragg, Robert T.
Pandya, Bhaumik
Atawia, Reem T.
Isales, Carlos
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-05-08
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Aging is associated with reduced muscle mass (sarcopenia) and poor bone quality (osteoporosis), which together increase the incidence of falls and bone fractures.
It is widely appreciated that aging triggers systemic oxidative stress, which can impair myoblast cell survival and differentiation.
We previously reported that arginase plays an important role in oxidative stress-dependent bone loss.
We hypothesized that arginase activity is dysregulated with aging in muscles and may be involved in muscle pathophysiology.
To investigate this, we analyzed arginase activity and its expression in skeletal muscles of young and aged mice.
We found that arginase activity and arginase 1 expression were significantly elevated in aged muscles.
We also demonstrated that SOD2, GPx1, and NOX2 increased with age in skeletal muscle.
Most importantly, we also demonstrated elevated levels of peroxynitrite formation and uncoupling of eNOS in aged muscles.
Our in vitro studies using C2C12 myoblasts showed that the oxidative stress treatment increased arginase activity, decreased cell survival, and increased apoptotic markers.
These effects were reversed by treatment with an arginase inhibitor, 2(S)-amino-6-boronohexanoic acid (ABH).
Our study provides strong evidence that L-arginine metabolism is altered in aged muscle and that arginase inhibition could be used as a novel therapeutic target for age-related muscle complications.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pandya, Chirayu D.& Lee, Byung& Toque, Haroldo A.& Mendhe, Bharati& Bragg, Robert T.& Pandya, Bhaumik…[et al.]. 2019. Age-Dependent Oxidative Stress Elevates Arginase 1 and Uncoupled Nitric Oxide Synthesis in Skeletal Muscle of Aged Mice. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1202321
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pandya, Chirayu D.…[et al.]. Age-Dependent Oxidative Stress Elevates Arginase 1 and Uncoupled Nitric Oxide Synthesis in Skeletal Muscle of Aged Mice. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1202321
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pandya, Chirayu D.& Lee, Byung& Toque, Haroldo A.& Mendhe, Bharati& Bragg, Robert T.& Pandya, Bhaumik…[et al.]. Age-Dependent Oxidative Stress Elevates Arginase 1 and Uncoupled Nitric Oxide Synthesis in Skeletal Muscle of Aged Mice. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1202321
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1202321
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر