Evaluation of Three Devices for the Isolation of the Stromal Vascular Fraction from Adipose Tissue and for ASC Culture: A Comparative Study
المؤلفون المشاركون
Rodriguez, Jonathan
Pratta, Anne-Sophie
Abbassi, Nacira
Fabre, Hugo
Rodriguez, Fanny
Debard, Cyrille
Adobati, Jacqueline
Boucher, Fabien
Mallein-Gerin, Frédéric
Auxenfans, Céline
Mojallal, Ali
Damour, O.
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-02-22
دولة النشر
مصر
عدد الصفحات
14
الملخص EN
Adipose-derived stem/stromal cells (ASCs) reside in the stromal vascular fraction (SVF) of adipose tissue (AT) and can be easily isolated.
However, extraction of the SVF from lipoaspirate is a critical step in generating ASC, and semiautomated devices have been developed to enhance the efficacy and reproducibility of the outcomes and to decrease manipulation and contamination.
In this study, we compared the reference method used in our lab for SVF isolation from lipoaspirate, with three medical devices: GID SVF-1™, Puregraft™, and Stem.pras®.
Cell yield and their viability were evaluated as well as their phenotype with flow cytometry.
Further on, we determined their proliferative potential using population doublings (PD), PD time (PDT), and clonogenicity assay (CFU-F).
Finally, we checked their genetic stability using RT-qPCR for TERT mRNA assay and karyotyping as well as their multilineage potential including adipogenic, chondrogenic, and osteogenic differentiation.
Our results demonstrate that all the devices allow the production of SVF cells with consistent yield and viability, in less time than the reference method.
Expanded cells from the four methods showed no significant differences in terms of phenotype, proliferation capabilities, differentiation abilities, and genetic stability.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rodriguez, Jonathan& Pratta, Anne-Sophie& Abbassi, Nacira& Fabre, Hugo& Rodriguez, Fanny& Debard, Cyrille…[et al.]. 2017. Evaluation of Three Devices for the Isolation of the Stromal Vascular Fraction from Adipose Tissue and for ASC Culture: A Comparative Study. Stem Cells International،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1202441
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rodriguez, Jonathan…[et al.]. Evaluation of Three Devices for the Isolation of the Stromal Vascular Fraction from Adipose Tissue and for ASC Culture: A Comparative Study. Stem Cells International No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1202441
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rodriguez, Jonathan& Pratta, Anne-Sophie& Abbassi, Nacira& Fabre, Hugo& Rodriguez, Fanny& Debard, Cyrille…[et al.]. Evaluation of Three Devices for the Isolation of the Stromal Vascular Fraction from Adipose Tissue and for ASC Culture: A Comparative Study. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1202441
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1202441
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر