Isolation and Characterization of Synovial Mesenchymal Stem Cell Derived from Hip Joints: A Comparative Analysis with a Matched Control Knee Group

الملخص EN

Purpose.

To determine the characteristics of MSCs from hip and compare them to MSCs from knee.

Methods.

Synovial tissues were obtained from both the knee and the hip joints in 8 patients who underwent both hip and knee arthroscopies on the same day.

MSCs were isolated from the knee and hip synovial samples.

The capacities of MSCs were compared between both groups.

Results.

The number of cells per unit weight at passage 0 of synovium from the knee was significantly higher than that from the hip (P<0.05).

While it was possible to observe the growth of colonies in all the knee synovial fluid samples, it was impossible to culture cells from any of the hip samples.

In adipogenesis experiments, the frequency of Oil Red-O-positive colonies and the gene expression of adipsin were significantly higher in knee than in hip.

In osteogenesis experiments, the expression of COL1A1 and ALPP was significantly less in the knee synovium than in the hip synovium.

Conclusions.

MSCs obtained from hip joint have self-renewal and multilineage differentiation potentials.

However, in matched donors, adipogenesis and osteogenesis potentials of MSCs from the knees are superior to those from the hips.

Knee synovium may be a better source of MSC for potential use in hip diseases.