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NOX2-Dependent Reactive Oxygen Species Regulate Formyl-Peptide Receptor 1-Mediated TrkA Transactivation in SH-SY5Y Cells
المؤلفون المشاركون
Cattaneo, Fabio
Ammendola, Rosario
Castaldo, Martina
Zollo, Cristiana
Esposito, Gabriella
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-02
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Several enzymes are capable of producing reactive oxygen species (ROS), but only NADPH oxidases (NOX) generate ROS as their primary and sole function.
In the central nervous system, NOX2 is the major source of ROS, which play important roles in signalling and functions.
NOX2 activation requires p47phox phosphorylation and membrane translocation of cytosolic subunits.
We demonstrate that SH-SY5Y cells express p47phox and that the stimulation of Formyl-Peptide Receptor 1 (FPR1) by N-fMLP induces p47phox phosphorylation and NOX-dependent superoxide generation.
FPR1 is a member of the G protein-coupled receptor (GPCR) family and is able to transphosphorylate several tyrosine kinase receptors (RTKs).
This mechanism requires ROS as signalling intermediates and is necessary to share information within the cell.
We show that N-fMLP stimulation induces the phosphorylation of cytosolic Y490, Y751, and Y785 residues of the neurotrophin receptor TrkA.
These phosphotyrosines provide docking sites for signalling molecules which, in turn, activate Ras/MAPK, PI3K/Akt, and PLC-γ1/PKC intracellular cascades.
N-fMLP-induced ROS generation plays a critical role in FPR1-mediated TrkA transactivation.
In fact, the blockade of NOX2 functions prevents Y490, Y751, and Y785 phosphorylation, as well as the triggering of downstream signalling cascades.
Moreover, we observed that FPR1 stimulation by N-fMLP also improves proliferation, cellular migration, and neurite outgrowth of SH-SY5Y cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Castaldo, Martina& Zollo, Cristiana& Esposito, Gabriella& Ammendola, Rosario& Cattaneo, Fabio. 2019. NOX2-Dependent Reactive Oxygen Species Regulate Formyl-Peptide Receptor 1-Mediated TrkA Transactivation in SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1202483
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Castaldo, Martina…[et al.]. NOX2-Dependent Reactive Oxygen Species Regulate Formyl-Peptide Receptor 1-Mediated TrkA Transactivation in SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-17.
https://search.emarefa.net/detail/BIM-1202483
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Castaldo, Martina& Zollo, Cristiana& Esposito, Gabriella& Ammendola, Rosario& Cattaneo, Fabio. NOX2-Dependent Reactive Oxygen Species Regulate Formyl-Peptide Receptor 1-Mediated TrkA Transactivation in SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1202483
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1202483
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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