An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs
المؤلفون المشاركون
Liu, Huihui
Wang, Zhongying
Qian, Minfei
Wang, Qixuan
Wu, Hao
Huang, Zhiwu
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-02
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The prestin-based active process in the mammalian outer hair cells (OHCs) is believed to play a crucial role in auditory signal amplification in the cochlea.
Prestin belongs to an anion transporter family (SLC26A).
It is densely expressed in the OHC lateral plasma membrane and functions as a voltage-dependent motor protein.
Analog genes can be found in the genome of nonmammalian species, but their functions in hearing are poorly understood.
In the present study, we used the gerbil prestin sequence as a template and identified an analog gene in the bullfrog genome.
We expressed the gene in a stable cell line (HEK293T) and performed patch-clamp recording.
We found that these cells exhibited prominent nonlinear capacitance (NLC), a widely accepted assay for prestin functioning as a motor protein.
Upon close examination, the key parameters of this NLC are comparable to that conferred by the gerbil prestin, and nontransfected cells failed to display NLC.
Lastly, we performed patch-clamp recording in HCs of all three hearing organs in bullfrog.
HCs in both the sacculus and the amphibian papilla exhibited a capacitance profile that is similar to NLC while HCs in the basilar papilla showed no sign of NLC.
Whether or not this NLC-like capacitance change is involved in auditory signal amplification certainly requires further examination; our results represent the first and necessary step in revealing possible roles of prestin in the active hearing processes found in many nonmammalian species.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Zhongying& Qian, Minfei& Wang, Qixuan& Liu, Huihui& Wu, Hao& Huang, Zhiwu. 2020. An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs. Neural Plasticity،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1202666
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Zhongying…[et al.]. An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs. Neural Plasticity No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1202666
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Zhongying& Qian, Minfei& Wang, Qixuan& Liu, Huihui& Wu, Hao& Huang, Zhiwu. An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs. Neural Plasticity. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1202666
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1202666
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر