Analysis of Crucial Genes and Pathways Associated with Spared Nerve Injury-Induced Neuropathic Pain
المؤلفون المشاركون
Mao, Dong
Zhai, Huang
Zhao, Gang
Mi, Jingyi
Rui, Yongjun
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-21
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Purpose.
The study was aimed at elucidating the molecular mechanism underlying neuropathic pain induced by spared nerve injury (SNI).
Methods.
The microarray data of GSE30691 were downloaded from the Gene Expression Omnibus database, including sciatic nerve lesion samples at 3, 7, 21, and 40 days after SNI and sham control samples at 3, 7, and 21 days.
Differential analysis along with Mfuzz clustering analysis was performed to screen crucial clusters and cluster genes.
Subsequently, comprehensive bioinformatic analyses were performed, including functional enrichment analysis, protein-protein interaction (PPI) network and module analysis, and transcription factor- (TF-) gene and miRNA-target interaction predictions.
Moreover, the screened differentially expressed genes (DEGs) were corroborated using two other microarray datasets.
Results.
Three clusters with different change trends over time after SNI were obtained.
Protein kinase CAMP-activated catalytic subunit beta (Prkacb), complement C3 (C3), and activating transcription factor 3 (Atf3) were hub nodes in the PPI network, and fibroblast growth factor 9 (Fgf9) was found to interact with more TFs.
Prkacb and Fgf9 were significantly enriched in the MAPK signaling pathway.
Moreover, rno-miR-3583-5p was targeted by Fgf9, and rno-miR-1912-3p was targeted by neuregulin 1 (Nrg1).
Key genes like Nrg1 and Fgf9 in cluster 1, Timp1 in cluster 2, and Atf3 and C3 in cluster 3 were screened out after corroborating microarray data with other microarray data.
Conclusions.
Key pathways like the MAPK signaling pathway and crucial genes like Prkacb, Nrg1, Fgf9, Timp1, C3, and Atf3 may contribute to SNI-induced neuropathic pain development in rats.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mao, Dong& Zhai, Huang& Zhao, Gang& Mi, Jingyi& Rui, Yongjun. 2020. Analysis of Crucial Genes and Pathways Associated with Spared Nerve Injury-Induced Neuropathic Pain. Neural Plasticity،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1202814
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mao, Dong…[et al.]. Analysis of Crucial Genes and Pathways Associated with Spared Nerve Injury-Induced Neuropathic Pain. Neural Plasticity No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1202814
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mao, Dong& Zhai, Huang& Zhao, Gang& Mi, Jingyi& Rui, Yongjun. Analysis of Crucial Genes and Pathways Associated with Spared Nerve Injury-Induced Neuropathic Pain. Neural Plasticity. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1202814
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1202814
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر