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MicroRNA-25 Protects Smooth Muscle Cells against Corticosterone-Induced Apoptosis
المؤلفون المشاركون
Yang, Zhen
Zhang, Bin
Li, Dong
Tan, Wenfeng
Zhang, Gaoxing
Wei, Tianlu
Mo, Ziqing
Liu, Jinxue
Wei, Yidong
Zhang, Lukun
Webster, Keith A.
Wei, Jianqin
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-12
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background and Aims.
Vascular smooth muscle cells (VSMCs) are central components of atherosclerotic plaque.
Loss of VSMCs through apoptotic cell death can cause fibrous cap thinning, necrotic core formation, and calcification that may destabilize plaque.
Elevated glucocorticoid levels caused by psychological stress promote VSMC apoptosis and can exacerbate atherosclerosis in mice and humans.
Changes in the levels of antiapoptosis microRNA-25 (miR-25) have been linked with heart disease, inflammation, VSMC phenotype, oxidative stress, and apoptosis.
Here, we investigated the pathways and mechanisms of glucocorticoid-induced apoptosis of mouse VSMCs and the protective role of miR-25.
Methods.
Primary mouse VSMCs were cultured +/- corticosterone for 48 h.
Apoptosis, ROS, apoptotic protein activities, miR-25, MOAP1, a miR-25 target, and p70S6 kinase were quantified at intervals.
The roles of miR-25 were assessed by treating cells with lenti-pre-miR-25 and anti-miR-25.
Results.
VSMC apoptosis, caspase-3 activity, and Bax were increased by corticosterone, and cell death was paralleled by marked loss of miR-25.
Protection was conferred by pre-miR-25 and exacerbated by anti-miR-25.
Pre-miR-25 conferred reduced expression of the proapoptotic protein MOAP1, and the protective effects of pre-miR-25 were abrogated by overexpressing MOAP1.
The antiapoptotic effects of miR-25 were paralleled by inhibition of the p70S6K pathway, a convergence target for the survival signaling pathways, and protection by pre-miR-25 was abrogated by the p70S6k inhibitor rapamycin.
Conclusions.
MicroRNA-25 blocks corticosterone-induced VSMC apoptosis by targeting MOAP1 and the p70S6k pathway.
Therapeutic manipulation of miR-25 may reduce atherosclerosis and unstable plaque formation associated with chronic stress.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Bin& Zhang, Gaoxing& Wei, Tianlu& Yang, Zhen& Tan, Wenfeng& Mo, Ziqing…[et al.]. 2019. MicroRNA-25 Protects Smooth Muscle Cells against Corticosterone-Induced Apoptosis. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1202836
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Bin…[et al.]. MicroRNA-25 Protects Smooth Muscle Cells against Corticosterone-Induced Apoptosis. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1202836
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Bin& Zhang, Gaoxing& Wei, Tianlu& Yang, Zhen& Tan, Wenfeng& Mo, Ziqing…[et al.]. MicroRNA-25 Protects Smooth Muscle Cells against Corticosterone-Induced Apoptosis. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1202836
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1202836
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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