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H-Ferritin Affects Cisplatin-Induced Cytotoxicity in Ovarian Cancer Cells through the Modulation of ROS
المؤلفون المشاركون
Costanzo, Francesco S.
Di Cello, Annalisa
Di Vito, Anna
Salatino, Alessandro
Aversa, Ilenia
Battaglia, Anna Martina
Sacco, Alessandro
Santamaria, Gianluca
Chirillo, Roberta
Veltri, Pierangelo
Tradigo, Giuseppe
Biamonte, Flavia
Venturella, Roberta
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-10-31
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Reactive oxygen species (ROS) mediates cisplatin-induced cytotoxicity in tumor cells.
However, when cisplatin-induced ROS do not reach cytotoxic levels, cancer cells may develop chemoresistance.
This phenomenon can be attributed to the inherited high expression of antioxidant protein network.
H-Ferritin is an important member of the antioxidant system due to its ability to store iron in a nontoxic form.
Altered expression of H-Ferritin has been described in ovarian cancers; however, its functional role in cisplatin-based chemoresistance of this cancer type has never been explored.
Here, we investigated whether the modulation of H-Ferritin might affect cisplatin-induced cytotoxicity in ovarian cancer cells.
First, we characterized OVCAR3 and OVCAR8 cells for their relative ROS and H-Ferritin baseline amounts.
OVCAR3 exhibited lower ROS levels compared to OVCAR8 and greater expression of H-Ferritin.
In addition, OVCAR3 showed pronounced growth potential and survival accompanied by the strong activation of pERK/pAKT and overexpression of c-Myc and cyclin E1.
When exposed to different concentrations of cisplatin, OVCAR3 were less sensitive than OVCAR8.
At the lowest concentration of cisplatin (6 μM), OVCAR8 underwent a consistent apoptosis along with a downregulation of H-Ferritin and a consistent increase of ROS levels; on the other hand, OVCAR3 cells were totally unresponsive, H-Ferritin was almost unaffected, and ROS amounts met a slight increase.
Thus, we assessed whether the modulation of H-Ferritin levels was able to affect the cisplatin-mediated cytotoxicity in both the cell lines.
H-Ferritin knockdown strengthened cisplatin-mediated ROS increase and significantly restored sensitivity to 6 μM cisplatin in resistant OVCAR3 cells.
Conversely, forced overexpression of H-Ferritin significantly suppressed the cisplatin-mediated elevation of intracellular ROS subsequently leading to a reduced responsiveness in OVCAR8 cells.
Overall, our findings suggest that H-Ferritin might be a key protein in cisplatin-based chemoresistance and that its inhibition may represent a potential approach for enhancing cisplatin sensitivity of resistant ovarian cancer cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Salatino, Alessandro& Aversa, Ilenia& Battaglia, Anna Martina& Sacco, Alessandro& Di Vito, Anna& Santamaria, Gianluca…[et al.]. 2019. H-Ferritin Affects Cisplatin-Induced Cytotoxicity in Ovarian Cancer Cells through the Modulation of ROS. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1203228
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Salatino, Alessandro…[et al.]. H-Ferritin Affects Cisplatin-Induced Cytotoxicity in Ovarian Cancer Cells through the Modulation of ROS. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1203228
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Salatino, Alessandro& Aversa, Ilenia& Battaglia, Anna Martina& Sacco, Alessandro& Di Vito, Anna& Santamaria, Gianluca…[et al.]. H-Ferritin Affects Cisplatin-Induced Cytotoxicity in Ovarian Cancer Cells through the Modulation of ROS. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1203228
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1203228
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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