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Fetal Rhesus D Genotyping and Sex Determination from Maternal Plasma of Rhesus D-Negative Antenatal Population: The Usefulness of Conventional Polymerase Chain Reaction in Resource-limited Settings
المؤلفون المشاركون
Obirikorang, Christian
Annani-Akollor, Max Efui
Duneeh, Richard Vikpebah
Amponsah, Francis A.
Afriyie, Edward Y.
Sakyi, Samuel Asamoah
Addai-Mensah, Otchere
Owiredu, Eddie-Williams
Asamoah Adu, Evans
المصدر
Obstetrics and Gynecology International
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-10-16
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Background.
This prospective cohort study evaluated the usefulness of conventional PCR in genotyping fetal Rhesus D (RhD) and sex from the maternal plasma of RhD-negative (RhD−) antenatal population in resource-limited settings.
Methods.
Thirty apparently healthy RhD− pregnant women with RhD positive (RhD+) partners were included.
Blood samples were collected from each participant (in the third trimester of pregnancy) for DNA extraction/purification and fetal RhD genotyping.
Results.
Out of the 30 samples, 26 (86.7%) were found to be RhD+ while 4 (13.3%) were RhD−.
The RhD+ comprised 24 (80.0%) RhD+ based on exons 5, 7, and 10 combined.
Exons 5 and 7 were detected in two additional samples but not exon 10.
Serological phenotyping of neonatal blood confirmed 26 RhD+ and 4 RhD−.
There was a perfect agreement between the fetal RhD genotype and neonatal RhD phenotyping after delivery for exons 5 and 7 (concordance = 100%, κ = 100.0%, diagnostic accuracy = 100%, p<0.0001) while exon 10 presented with an almost perfect agreement (concordance = 93.3%, κ = 76.2%, diagnostic accuracy = 93.3%, p<0.0001).
Regarding the prenatal test for the SRY gene, 9 (30.0%) were predicted to be males and the remaining 21 (60.0%) were females.
All the 9 and 21 anticipated males and females, respectively, were confirmed after delivery (concordance = 100%, κ = 100.0%, diagnostic accuracy = 100%).
Conclusion.
Our study suggests that conventional PCR using the SRY, RhD exons 5 and 7 could be useful for predicting fetal sex and RhD from maternal peripheral blood in resource-limited settings.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Addai-Mensah, Otchere& Afriyie, Edward Y.& Sakyi, Samuel Asamoah& Obirikorang, Christian& Annani-Akollor, Max Efui& Owiredu, Eddie-Williams…[et al.]. 2020. Fetal Rhesus D Genotyping and Sex Determination from Maternal Plasma of Rhesus D-Negative Antenatal Population: The Usefulness of Conventional Polymerase Chain Reaction in Resource-limited Settings. Obstetrics and Gynecology International،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1203334
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Addai-Mensah, Otchere…[et al.]. Fetal Rhesus D Genotyping and Sex Determination from Maternal Plasma of Rhesus D-Negative Antenatal Population: The Usefulness of Conventional Polymerase Chain Reaction in Resource-limited Settings. Obstetrics and Gynecology International No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1203334
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Addai-Mensah, Otchere& Afriyie, Edward Y.& Sakyi, Samuel Asamoah& Obirikorang, Christian& Annani-Akollor, Max Efui& Owiredu, Eddie-Williams…[et al.]. Fetal Rhesus D Genotyping and Sex Determination from Maternal Plasma of Rhesus D-Negative Antenatal Population: The Usefulness of Conventional Polymerase Chain Reaction in Resource-limited Settings. Obstetrics and Gynecology International. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1203334
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1203334
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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