Aloin Preconditioning Attenuates Hepatic IschemiaReperfusion Injury via Inhibiting TLR4MyD88NF-κB Signal Pathway In Vivo and In Vitro
المؤلفون المشاركون
Du, Yichao
Qian, Baolin
Gao, Lin
Tan, Peng
Chen, Hao
Wang, Ankang
Zheng, Tianxiang
Pu, Shilin
Xia, Xianming
Fu, Wenguang
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-20
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Background.
Aloin exerts considerable protective effects in various disease models, and its effect on hepatic ischemia-reperfusion (HIR) injury remains unknown.
This research is aimed at conducting an in-depth investigation of the antioxidant, anti-inflammatory, and antiapoptosis effects of aloin in HIR injury and explain the underlying molecular mechanisms.
Methods.
In vivo, different concentrations of aloin were intraperitoneally injected 1 h before the establishment of the HIR model in male mice.
The hepatic function, pathological status, oxidative stress, and inflammatory and apoptosis markers were measured.
In vitro, aloin (AL, C21H22O9) or lipopolysaccharide (LPS) was added to a culture of mouse primary hepatocytes before it underwent hypoxia/reoxygenation (H/R), and the apoptosis in the mouse primary hepatocytes was analyzed.
Results.
We found that 20 mg/kg was the optimum concentration of aloin for mitigating I/R-induced liver tissue damage, characterized by decreased serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST).
Aloin pretreatment substantially suppressed the generation of hepatic malondialdehyde (MDA), tumor necrosis factor alpha (TNF-α), and IL-6 and enhanced the hepatic superoxide dismutase (SOD) activities as well as glutathione (GSH) and IL-10 levels in the liver tissue of I/R mice; this indicated that aloin ameliorated I/R-induced liver damage by reducing the oxidative stress and inflammatory response.
Moreover, aloin inhibited hepatocyte apoptosis and inflammatory response that was caused by the upregulated expression of Bcl-2, the downregulated expression of cleaved caspase3(C-caspase3), Bax, Toll-like receptor 4 (TLR4), FADD, MyD88, TRAF6, phosphorylated IKKα/β (p-IKKα/β), and phosphorylated nuclear factor κB p65 (p-NF-κB p65).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Du, Yichao& Qian, Baolin& Gao, Lin& Tan, Peng& Chen, Hao& Wang, Ankang…[et al.]. 2019. Aloin Preconditioning Attenuates Hepatic IschemiaReperfusion Injury via Inhibiting TLR4MyD88NF-κB Signal Pathway In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1203395
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Du, Yichao…[et al.]. Aloin Preconditioning Attenuates Hepatic IschemiaReperfusion Injury via Inhibiting TLR4MyD88NF-κB Signal Pathway In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1203395
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Du, Yichao& Qian, Baolin& Gao, Lin& Tan, Peng& Chen, Hao& Wang, Ankang…[et al.]. Aloin Preconditioning Attenuates Hepatic IschemiaReperfusion Injury via Inhibiting TLR4MyD88NF-κB Signal Pathway In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1203395
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1203395
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر